肿瘤坏死因子α和β对电离辐射诱导细胞凋亡的效应  

EFFECT OF TUMOR NECROSIS FACTOR α AND β ON IONIZING RADIATION-INDUCED APOTOSIS

作　　者：曹明富[1] 应蓓蓓[2] 徐人尔[2] 赵寿元[2] 李昌本[2] 

机构地区：[1]杭州师范学院生物学系,杭州310036 [2]复旦大学遗传学研究所遗传工程国家重点实验室,上海200433

出　　处：《实验生物学报》2002年第3期198-204,共7页Acta Biologiae Experimentalis Sinica

基　　金：浙江省"151人才工程"基金;杭州市"131人才工程"基金资助

摘　　要：为探讨肿瘤坏死因子(tumor necrosis foctor)α和β(TNFα和β)对电离辐射诱发细胞凋亡的效应及其机理,采用DNA琼脂糖凝胶电泳和FACS分析等方法,观察了人肿瘤坏死因子α(hTNFα)和β(hTNFβ)对^(60)Co-γ射线诱发细胞凋亡的形态学,生化学变化。结果显示:hTNFα或hTNFβ均可明显抑制^(60)Co-γ射线诱发正常人胚肺二倍体细胞(2BS)的凋亡,而相同剂量的hTNFα能促进^(60)Co-γ射线诱发的人体肺腺癌细胞系A549细胞凋亡,而对另一株人体肺癌SPC细胞的效应比A549降低1倍;hTNFβ能分别增强A549和SPC的细胞凋亡频率。由此认为,hTNFα和hTNFβ均可通过调节细胞的生理生化反应来改变细胞对电离辐射的敏感性,可保护正常细胞免受辐射损伤,而增加某些肿瘤细胞对辐射的敏感性。Present paper described the effect of human tumor necrosis factor α (hTNFα)and β (hTNFβ) on apoptosis induced by ionizing radiation in human embryonic lung cell line 2BS diploid cells and human lung cancer cell line A549 and SPC cells. The results showed that both hTNFα and hTNFβ significantly inhibited 7Gy γ-ray-induced apoptosis in 2BS cells. In contrast, hTNFα or hTNFβ can increase the sensitivity of tumor cell lines (A549 and SPC cells) to radiation. Therefore, the results suggested that TNFα and TNFβ had potential as a therapeutic agent to protect normal cell from the radiation-induced apoptosis and to sensitize the tumour cells for the damage effect of ionizing radiation.

关 键 词：肿瘤坏死因子Α 电离辐射 诱导 肿瘤坏死因子Β 细胞凋亡 淋巴毒素 辐射敏感性 辐射防护 肿瘤细胞 

分 类 号：R363[医药卫生—病理学]