液相色谱-紫外检测法测定蜂蜜中的β-呋喃果糖苷酶活性  

Determination of β-fructofuranosidaseActivity in Honey by HPLC-UV

作　　者：张其安 张庆娜[2] 吕祝章[2] 卢德梅 杨少波 

机构地区：[1]山东华康蜂业有限公司,日照276500 [2]日照职业技术学院,日照276826

出　　处：《中国蜂业》2012年第Z2期92-96,共5页Apiculture of China

摘　　要：建立了一种高效液相色谱法测定蜂蜜中β-呋喃果糖苷酶(β-fructofuranosidase)活性的方法,以浓度为10%的蜜三糖为反应底物,对氨基苯甲酸(p-AMBA)衍生化后,紫外检测法测蜜二糖的生成量,计算β-呋喃果糖苷酶活性。结果表明:该方法检出限为10U/kg,线性范围为20～240U/kg,r≥0.9988;回收率为85.6%～115.5%,RSD为2.2%～4.5%,该方法准确、灵敏,适用于蜂蜜中β-呋喃果糖苷酶活性的测定。The HPLC method with 10% raffinose as the enzyme reaction substrate was established.p-AMBA was used for pre-column derivatization of the analytes.The hydrolysis activity of β-fructofuranosidase could be accurately calculated by test the contens of melibiose.The results showed that the detection limit of β-fructofuranosidase Activity is 10U/kg,linear ranges is 20～240 U/kg.The spiked recoveris is in the range of 85.6%～115.5%,RSD is in the range of 2.2%～4.5%.This method is accurate and sensitive.It can be used for determination of β-fructofuranosidase activity in honey.

关 键 词：高效液相色谱 紫外检测 蜂蜜 Β-呋喃果糖苷酶 

分 类 号：S896.1[农业科学—特种经济动物饲养]