蜜蜂壳聚糖提取与抑菌研究  

Studies on honeybee chitosan extraction and its inhibitory effect on bacteria

作　　者：王丽华[1] 陈婉玉[1] 王芳[2] 邱俊[1] 丛明辰 

机构地区：[1]福建农林大学蜂学学院 [2]国家教育部蜂产品加工与应用工程研究中心,福建农林大学蜂学学院,福州金山350002

出　　处：《中国蜂业》2012年第Z4期33-38,共6页Apiculture of China

基　　金：福建农林大学2007-2008年大学生创新性实验项目

摘　　要：壳聚糖具有优良的抑菌特性,已广泛应用于与生活密切相关的各个领域。对壳聚糖的提取研究和生产,目前仅局限于蟹壳、虾壳、蚕蛹和蝇蛹等,蜜蜂壳聚糖的提取研究尚未见报道。用酸碱法从蜜蜂体壁中去除无机盐和有机物后,再在甘油浴中进行一定时间梯度的脱乙酰基反应。所得可溶性蜜蜂壳聚糖用来进行对蜜蜂球囊菌和大肠杆菌的抑菌实验以及对蜜蜂的饲喂实验。蜜蜂甲壳素脱乙酰度以其在碱液中加热50min为最好。可溶性壳聚糖对蜜蜂球囊菌(蜜蜂白垩病致病菌)没有抑制作用,但对大肠杆菌有很好的抑制作用。以可溶性壳聚糖溶解蜂花粉后饲喂蜜蜂,蜜蜂少取食或不取食。Chitosan has excellent antibacterial properties, has been widely used in life and is closely related to various fields of.On chitosan extraction research and production, is currently limited to crab, shrimp, silkworm chrysalis and fly pupae, bee chitosan extraction research has not been reported.The acid-base method from bee wall removal of inorganic salts and organic compounds, and then in glycerin bath for a certain time gradient of deacetylation reaction.The soluble bee chitosan used for Ascosphaera apis and Escherichia coli bacteriostasis experiment and the bees feeding experiment.Bee deacetylation degree with its in alkaline solution as the best heating 50min.Soluble chitosan on Ascosphaera apis ( bee chalkbrood pathogen ) without inhibition on Escherichia coli, but has good inhibiting effect.Soluble chitosan dissolved bee pollen feeding bees, bee feeding or do not eat less.

关 键 词：蜜蜂 甲壳素 壳聚糖 蜜蜂球囊菌 大肠杆菌 

分 类 号：S895.137[农业科学—特种经济动物饲养]