叶绿素显示液对淋巴结显色的实验及临床应用观察  

The Experimental and Clinical Study on Chlorophyll Solution Staining Lymph Nodes

作　　者：王新明[1] 杨洪霞[2] 解家英[2] 王海杰[3] 鞠学红[1] 

机构地区：[1]潍坊医学院解剖学教研室,山东省潍坊市261042 [2]潍坊市人民医院 [3]复旦大学医学院

出　　处：《中国肿瘤临床》2004年第18期17-19,共3页Chinese Journal of Clinical Oncology

摘　　要：目的：探讨叶绿素显示液在妇科肿瘤淋巴结清扫术中的应用价值。方法：采用１ ０ 只狗睾丸实质或子宫角内注射叶绿素显示液后，观察盆腔内淋巴结的显色情况，并在叶绿素注射后不同的时间取显色淋巴结进行光、电镜观察，股静脉采血对叶绿素浓度进行定量测量。研究组经子宫颈注射叶绿素显示液，对切除的淋巴结作石蜡切片和冰冻切片，显微镜下观察。结果：光镜下叶绿素进入淋巴结的被膜下窦和小梁周窦；电镜下叶绿素注射２ h 后，淋巴结被膜下窦内有少量叶绿素颗粒，髓质巨细胞内、小血管内亦见叶绿素颗粒。４ h 后，髓质巨细胞内显色颗粒最大，随后，颗粒密度逐渐降低。血液浓度在叶绿素注射后２ h 为９ ２ ．３ ６ 滋g／m l，４ h 最高为３ ６ ０ ．６ ５ 滋g／m l，２４ h 后叶绿素在血液内消失。研究组淋巴结切除率明显高于对照组，切除淋巴结的时间短于对照组。结论：经子宫颈注射叶绿素，能有效显示盆腔淋巴结，可提高淋巴结的清除率及避免损伤周围组织。Objective: To detect the significance of chlorophyll disclosing solution in lymphadenectomy of female malignant tumors. Methods: In 10 dogs, chlorophyll solution was injected into the testis or theuterine cornu. The pelvic cavity lymph nodes were observed by naked eyes, light microscope and electron microscope, the concentration of chlorophyll in the blood was measured. The chlorophyll solution was injected into the cervix in the coloring group. Paraffin sections and freeze sections of the resected nodes were made. Results: The chlorophyll granules were found in lymphoid sinus under light microscope. Four hrs after injection many chlorophyll granules were seen within macrophages under electron microscope, but the density of chlorophyll granules decreased. the concentration of chlorophyll in the blood was 92.36?滋g/ml and 360.65?滋g/ml at the 2nd and 4th hour respectively. The rates of the resected nodes in study group were higher than those in control group significantly. Duration of resection in study group was shorter than that in control group. Conclusion: Injection of chlorophyll into cervix before lymphadenectomy was useful for lymph nodes resection, operation time and avoiding adjacent structures.

关 键 词：叶绿素 淋巴结 盆腔 宫颈癌 

分 类 号：R737.3[医药卫生—肿瘤]