Activated focal adhesion kinase involved in adhesion and migration of vas cular smooth muscle cells stimulated by fibronectin  

粘着斑激酶活化对平滑肌细胞粘附和迁移的影响(英文)

作　　者：尹航[1] 汪丽蕙[1] 霍勇[1] 彭旭[1] 夏春芳[1] 唐朝枢[1] 

机构地区：[1]北京大学第一医院心脏科,北京100034

出　　处：《Chinese Medical Journal》2002年第4期14-17,145-146,共6页中华医学杂志（英文版）

摘　　要：To study the effects of focal adhesion kinase (FAK) phosphorylation on smooth mu scle cells (SMCs) adhesion and migration stimulated by fibronectin Methods Adhesion and migration of cultured SMCs were stimulated by different concentrati ons of fibronectin (FN), FAK and its phosphorylation were detected by immunoprec ipitation and Western blot FAK antisense oligodeoxynucleotides (ODNs) were tra nsfected into SMCs by cationic lipid to investigate its modulatory effects on ty rosine phosphorylation SMCs adhesion and migration were also measured by morph ological enumeration and modified Boyden Chambers, respectively Results FAK were expressed when SMCs adhesion and migration were successfully simulated by different concentrations of FN FAK phosphorylation were detected only at 20 ?μg/ml FN or more FAK antisense ODNs were transfected efficiently by cationi c lipid and FAK phosphorylation was inhibited substantially The SMCs migration rate in the 5-60?μg/ml FN groups was reduced by 17 89%-27 67% Cell migrat ion stimulated by FN at 10, 20, 40 and 60?μg/ml were reduced by 23 26%, 21 6 3%, 19 31% and 17 88%, respectively ( P 【0 05) Conclusions FAK phosphorylation and FAK mediated signal transduction play important roles i n SMCs adhesion and migration stimulated by ECM The process can be inhibited e ffectively by FAK antisense ODNs目的　研究粘着斑激酶磷酸化在细胞外基质成份诱导平滑肌细胞粘附和迁移中的作用。方法　通过纤粘连蛋白 (fibronectin ,FN)诱导培养的平滑肌细胞粘附迁移 ,以免疫沉淀和Westernblot方法检测粘着斑激酶 (focaladhesionkinase ,FAK)及其磷酸化的表达量。将FAK反义寡核苷酸 (antisenseoligodeoxynucleotides ,ODNs)经脂质体转染细胞 ,观察对FAK磷酸化、细胞粘附铺展和迁移的影响。结果　FN在显著诱导平滑肌细胞粘附和迁移时 ,FAK也呈明显表达 ,2 0 μg/mlFN可使其磷酸化处于较高表达量。脂质体可有效地介导ODNs转染 ,转染效率为 86 7%± 4 5 % ,FAK磷酸化表达量明显减少 ,5 - 6 0 μg/ml不同浓度FN组 ,细胞铺展率减少 17 89% - 2 7 6 7% ,10、 2 0、 4 0和 6 0 μg/mlFN组迁移细胞数也分别显著减少2 3 2 6 %、2 1 6 3%、19 31%、17 88% (P <0 0 5 )。结论　活化的FAK是细胞外基质诱导SMCs粘附和迁移的重要信号分子 ,由其介导的信号转导促进了这一过程 ,反义FAKODNs可有效地对此进行抑制。

关 键 词：focal adhesion kinase    · vascular smooth muscle  cells  ·   antisense oligodeoxynucleotides  ·   adhesion  ·    migrat ion 

分 类 号：R[医药卫生]