叶酸缺乏的离乳大鼠模型建立和评价  

THE ESTABLISHMENT & EVALUATION OF FOLATE DEFICIENCY MODEL OF MEANLING RATS.

作　　者：杨慧敏[1] 林茹珠[2] 廖国仪[1] 周冬[1] 孙轶[1] 黄云祖[1] 陈觉凝[1] 

机构地区：[1]暨南大学医学院第一附属医院儿科,510632 [2]中山市人民医院儿科,528400

出　　处：《中国小儿血液》2002年第4期161-166,共6页China Child Blood

基　　金：广东省自然科学基金资助项目(980 70 0 )

摘　　要：初次探讨在国内尝试用RHAA配方建立离乳大鼠的FD模型 ,并对该模型进行评价 ,以期今后对叶酸缺乏 (FolatesDeficiency ,FD)影响离乳大鼠学习记忆的观察和可能机制的分析有一更可信的基础。选择 5 4只刚离乳的健康雄性SD大鼠 ,随机分成实验组、对饲组和对照组三组。实验组饲以去叶酸的RHAA配方饲料 ,另二组饲以添加了 8mg叶酸 /kg膳食的RHAA饲料 ,对饲组按实验组的进食量加以控制 ,对照组不控制进食。每周记录体重和摄食量两次。分别于饲养第 2和第 6周末测定血清叶酸浓度。结果 :实验第 2周除实验组血清叶酸浓度降低外 (P <0 .0 1) ,其余各指标在三组间无明显差异 (P >0 .0 5 )。实验组从第 3周、对饲组从第 4周开始摄食量减少 ,生长速度减慢 (P <0 .0 5 )。第 6周实验组的平均体重、血清叶酸浓度明显低于对照组和对饲组 (P <0 .0 1)。结论显示 :用RHAA配方饲料饲养 6周可建成较重的离乳大鼠的叶酸缺乏模型 ,并最大限度地促进其生长 。The FD (folate deficiency) model of weaning rats fed with amino acid-defined diet (RHAA) was preliminarily established and evaluated. It was expected to provide basic zoological evidence in study of FD's effect to weanling rat's learning & memory. 54 male weanling Sprague-Dawley rats were divided into three groups. The folate deficient group received an amino acid-defined diet (RHAA), the second group had the same diet supplemented with folate of 8mg per kg diet, and the pair-fed control group received the folate supplemented diet, but was individually pair-fed to the mean daily food consumption of the folate deficient group. Body weight and food intake were measured twice weekly. The serum folate concentration were measured. Results showed Growth retardation and impaired food intake associated with FD could not be demonstrated in the FD group rats until the end of the 3 rd week ( P< 0.05), while the pair-fed group gradually showed this retardation at the end of the 4 th week. At the end of the experiment the FD rats had weight loss, leaving the pair-fed group slightly affected (compared with ad libitum control group, P< 0.05). Decreased serum folate occurred at the end of the 2 nd week of the experiment. At the end of the 6 th week, FD group rats had lower body weight and serum folate concentration( P< 0.01). Therefore, folate deficiency could be produced in 6 weeks with refined amino acid diets that promoted maximum growth rate, it's an effective protocol to produce experimental FD in young animals within a short period of time.

关 键 词：叶酸缺乏 离乳大鼠 模型建立 评价 

分 类 号：R-332[医药卫生]