HeLa细胞HSP-70体外诱导抗瘤免疫活性细胞的研究  

Specific Anti-tumor Effect of Immune Competent Cell Inducedby HSP-70 of HeLa Cells In Vitro

作　　者：韩彩芝[1] 刘京生[1] 许顺江[1] 郑秀芳[2] 魏丽君[1] 

机构地区：[1]河北省医学科学院免疫研究室,河北石家庄050021 [2]石家庄市人民医院,河北石家庄050000

出　　处：《癌症》2002年第10期1062-1064,共3页Chinese Journal of Cancer

基　　金：河北省自然科学基金项目(项目编号:398332)

摘　　要：背景与目的:近来的研究证实,动物肿瘤细胞表达的热休克蛋白(heatshokproteinHSP)可携带肿瘤抗原,并能诱导抗瘤特异性T淋巴细胞的免疫应答。本文旨在探讨用HeLa细胞HSP-70多肽诱导的免疫活性细胞(immunecompetentcell,ICC)及其特异性抗肿瘤机制。方法:用热休克处理的HeLa细胞提取的HSP-70多肽剌激正常人外周血单个核细胞(PBMCs),在剌激扩增前后测定T淋巴细胞表型,并测定扩增的免疫活性细胞对HeLa细胞的杀瘤活性。结果:在扩增前后CD3+淋巴细胞百分率为(57.68±1.46)%和(85.73±1.44)%(P<0.002),CD8+淋巴细胞百分率为(23.56±1.86)%和(48.06±1.66)%(P<0.002)。该免疫活性细胞对HeLa细胞和来源于病人的宫颈癌细胞都具有较高的杀伤活性犤(82.69±1.97)%和(62.11±1.61)%犦,而对经HSP-70单抗封闭后的HeLa细胞的杀伤活性犤(31.05±2.09)%犦则明显下降。结论:HeLa细胞HSP-70多肽能有效刺激PBMCs诱导活化免疫活性细胞,该免疫活性细胞具有特异性抗肿瘤作用。Background & Objective: Recent studies indicated that heat shock proteins (HSPs) are associated with antigenic peptides and participate in the antitumor T cell response in animals.This study was designed to investigate the mechanism of anti tumor effect of immune competent cells (ICC) induced by HSP 70 of HeLa ( cervical carcinoma ) cells in vitro. Methods:The normal peripheral blood mononuclear cells(PBMCs) stimulated with HSP 70 of HeLa cells in vitro.T cell phenotypes were analyzed before and after stimulated by PBMCs. HSP 70 primed the immune competent cells (ICCs) from PBMCs were tested for cytotoxic activity against HeLa cell. Results: CD3+ cell was 85.73±1.44%,up from 57.68±1.46% before the amplification(P< 0.002).CD8+ cell was 48.06±1.66%,up from 23.56±1.86% before the amplification(P< 0.002). The ICCs induced by HSP 70 were shown significant cytotoxic activity (82.69±1.97%,62.11±1.61%)against HeLa cells and cervical carcinoma cells,and the cytotoxic activity (31.05±2.09%)of the ICC against HeLa cells could be blocked by anti HSP 70 antibody. Conclusion: The ICC can be induced selectively by PBMCs with HSP 70 of HeLa cells, and has specific cytotoxic activity against HeLa cell.

关 键 词：HELA细胞 热休克蛋白-70 免疫活性细胞 ICC 特异性抗肿瘤作用 免疫应答 T淋巴细胞 

分 类 号：R730.3[医药卫生—肿瘤]