不同类型胶质瘤基因表达谱的初步分析  

Preliminary Analysis on the Gene Expression Profiles of Different Types of Gliomas with cDNA Array

作　　者：江荣才[1] 浦佩玉[1] 许杰[2] 于士柱[3] 焦保华 郭政[2] 李霞[5] 康春生[1] 

机构地区：[1]天津医科大学总医院神经外科,天津300052 [2]哈尔滨医科大学生物信息研究室,黑龙江哈尔滨150086 [3]天津医科大学总医院神经病理室,天津300052 [4]河北省第二医院神经外科,河北石家庄050000 [5]哈尔滨医科大学医学数学研究室,黑龙江哈尔滨150086

出　　处：《癌症》2002年第10期1085-1089,共5页Chinese Journal of Cancer

基　　金：国家自然科学基金项目(No.30070774;No.30170515;No.39970397)

摘　　要：背景与目的:区分不同类型胶质瘤的分子发病机制具有重要的临床和生物学意义。基因芯片可揭示肿瘤基因表达谱,可能成为肿瘤分子病理学的新工具。我们拟应用cDNA微阵列技术来分析不同类型胶质瘤的基因表达谱差异。方法:提取来自4例脑胶质瘤患者3种类型的胶质瘤标本(包括少支细胞胶质瘤、间变性星形细胞瘤以及室管膜瘤)以及1例正常脑组织标本的总RNA,逆转录成32P标记的cDNA探针,与Atlas微阵列膜杂交、放射自显影,用专用软件和聚类分析法分析基因表达谱。结果:与正常脑组织相比,各肿瘤的差异表达基因数量从11到118个不等,选取107个差异表达基因作为聚类分析指标,可将4例胶质瘤分成3种类型且与神经病理学诊断相符。结论:使用微阵列技术可分辨不同类型胶质瘤的基因表达谱。Background & Objective:There is great signification to classify the molecular pathogenesis of gliomas. Gene chip is able to profile the gene expression of tumors, which may become a new method for studying molecular pathology of tumors. The cDNA was used in order to detect the differences in gene expression profiles of different types of gliomas. Methods:cDNA probes tagged with 32P were converted from total RNA extracted from 4 samples of 3 different pathological types,including oligodendrogliomas,anaplastic astrocytomas and ependymomas,and one sample of normal human brain tissue as well, which were then hybridized to the Atlas array. After autoradiography, the gene expression profiles were analyzed with the special software and by the cluster analysis. Results: As compared to the normal brain tissue, the number of differential expressed genes in these tumor samples ranging from 11 to 118 was found. These 4 samples could be divided into 3 kinds of types when 107 differential expressed genes were selected to be as the cluster analysis index. This result was in accordance with the neuropathological diagnosis. Conclusion:cDNA array is able to profile gene expression in different types of gliomas. Combined with the bioinformational method,we may further obtain useful information about molecular pathology of gliomas.

关 键 词：CDNA微阵列 少支胶质细胞瘤 星形细胞瘤 室管膜瘤 基因表达谱 

分 类 号：R739.41[医药卫生—肿瘤] Q78[医药卫生—临床医学]