可溶性重组人CD40配体抑制人胶质瘤细胞生长  

Inhibition of Human Glioma Cell Growth by a Soluble Recombinant Human CD40 Ligand

作　　者：高宜录[1] 时宏珍[2] 刘梅[2] 顾晓松[3] 

机构地区：[1]南通医学院附属医院神经外科,江苏南通226001 [2]南京医科大学附属南京第一医院中心实验室,江苏南京210006 [3]南通医学院江苏省神经生物学重点实验室,江苏南通226001

出　　处：《癌症》2002年第10期1112-1115,共4页Chinese Journal of Cancer

摘　　要：背景和目的:CD40是存在于B细胞、单核细胞、树突状细胞、内皮细胞和多种肿瘤细胞上的肿瘤坏死因子受体超家族成员,已有研究证明用CD40抗体或CD40配体激活CD40可引起人卵巢癌、结肠癌、乳腺癌和肺癌细胞凋亡或死亡,并抑制鼠体内移植瘤生长。本研究拟了解可溶性重组人CD40配体(srhCD40L)对人胶质瘤细胞增殖的影响。方法:用免疫组化法检测胶质瘤标本中CD40的表达。用流式细胞仪检测人胶质瘤细胞中CD40的表达,然后在其培养基中加入srhCD40L孵育,在分别培养72h和24h后检测细胞增殖和凋亡指标。结果:23个胶质瘤标本中有7个和3个胶质瘤细胞系有1个具有CD40表达。有CD40表达的胶质瘤细胞系与srhCD40L共孵育后发现细胞活力从98%降至87%;凋亡和坏死升高了17.4%。结论:在体外用CD40配体激活CD40可抑制有CD40表达的人胶质瘤细胞的生长,提示CD40配体在治疗胶质瘤上有潜在的临床应用价值。Background & Objective: CD40 presents on B cells, monocytes, dendritic cells, and endothelial cells, as well as a variety of neoplastic cell types,including carcinomas.CD40 stimulated by its antibody or CD40 ligand previously had been demonstrated to induce activation induced cell death in ovarian carcinoma cell lines, colon carcinoma cell lines, breast carcinoma cell lines,and lung carcinoma cell lines and to inhibit their growth in vivo.This study was designed to assess the effects of a recombinant soluble human CD40 ligand (srhCD40L) on human glioma cell lines. Methods: The specimens of human glioma were examined for CD40 expression by immunohistochemistry. The human glioma cell lines were examined for CD40 expression by flow cytometry. The cell lines were incubated with srhCD40L for 24h and 72h,then the markers of proliferation and apoptosis of these cells were determined, respectively. Results: CD40 expression could be detected in 7/23 human glioma specimens and 1/3 cell line. The srhCD40L inhibited the proliferation of CD40+ human glioma cell line. The viability of these cells was decreased from 98% to 87%. The apoptosis and death of these cells were increased by 17.4%. Conclusions: CD40 stimulated by its ligand directly inhibits human glioma cell growth in vitro. These results suggest that srhCD40L may be of clinic use to inhibit glioma growth.

关 键 词：CD40 CD40配体 胶质瘤 凋亡 免疫组化 

分 类 号：R739.41[医药卫生—肿瘤]