L-精氨酸和L-NAME对牛小梁细胞增殖和凋亡的影响  

Effect of L-arginine and L-NAME on proliferation and apoptosis of cultured bovine trabecular meshwork cells

作　　者：薛蔚[1] 杜蜀华[1] 李勇[2] 孙京华[1] 杨业金[3] 

机构地区：[1]华中科技大学附属同济医学院眼科,武汉430030 [2]华中科技大学附属协和医院普外科 [3]华中科技大学附属同济医学院分子生物学教研室,武汉430030

出　　处：《眼科研究》2002年第5期401-403,共3页Chinese Ophthalmic Research

摘　　要：目的　研究L 精氨酸和L NAME对体外培养的牛小梁细胞增殖和凋亡的影响。方法　在体外培养的牛小梁细胞中分别加入浓度为 1× 10 -7mol/L、1× 10 -6mol/L、1× 10 -5mol/L、1× 10 -4 mol/L、1× 10 -3 mol/L及 1× 10 -2 mol/L的L 精氨酸和左旋 -硝基精氨酸甲酯 (L NAME)与小梁细胞共同孵育 48h ,以不加药组为对照组 ,用化学比色法、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法 (TUNEL)及四甲基偶氮唑盐 (MTT)法 ,检测小梁细胞在上述药物中增殖和凋亡状况。结果　≥ 1× 10 -4 mol/L的L 精氨酸通过促进NO的产生导致小梁细胞凋亡并抑制细胞的增殖 ;而≥ 1× 10 -5mol/L的L NAME通过抑制NO的产生 ,促进小梁细胞的增殖。结论　高浓度的NO可抑制小梁细胞的增殖 ,诱导细胞凋亡。ObjectiveTo study the proliferation and apoptosis of cultured bovine trabecular meshwork(TM)cells influenced by L-arginine and L-NAME .MethodsTM cells with L-arginine and L-NAME were incubated for 48 h.In control group,no medicine was added.In treatment groups,concentrations of L-arginine and L-NAME were 1×10 -7 mol/L,1×10 -6 mol/L,1×10 -5 mol/L,1×10 -4 mol/L,1×10 -3 mol/L,1×10 -2 mol/L,respectively.NO 2 -in supernate,the proliferation and apoptosis of TM cells were detected by Griess assay,terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and MTT assay.Result≥1×10 -4mol/L of L-arginine could induce the apoptosis of TM cells and inhibit the proliferation of TM cells by promoting the synthesis of NO.However,≥1×10 -5mol/L of L-NAME could accelerate the proliferation of TM cells by inhibiting NO product.Conclusion Nitric oxide in high concentration can induce TM cell apoptosis and suppress cell proliferation.

关 键 词：L-精氨酸 L-NAME 小梁细胞 一氧化氮 增殖 凋亡 

分 类 号：R770.2[医药卫生—眼科]