脑缺血对昆明小鼠大脑动脉环结构的影响  

Effect of cerebral ischemia on Willis cycle in the Kunming mouse

作　　者：金道忠[1] 刘正清[1] 马志健[1] 张志君[1] 蔡维君[1] 文晓丹[1] 

机构地区：[1]中南大学湘雅医学院人体解剖学教研室

出　　处：《解剖学研究》2002年第3期175-176,共2页Anatomy Research

基　　金：湖南省自然科学基金资助项目 (OOJJY2 0 69)

摘　　要：目的　探讨昆明小鼠是否具有大脑动脉环 (Willis环 )结构。方法　结扎昆明小鼠双侧颈总动脉 ,经升主动脉灌注甲苯胺蓝 ,观察脑染色区域。结扎双侧颈总动脉 4、7和 10min ,存活 4 8h后观察海马CA1区神经元Caspase 3的表达。结果　双侧颈总动脉结扎后 ,双侧大脑半球未染色 ,而小脑、脑干染色 ,双侧海马冠状切面均未染色 ,而对照组全脑染色。结扎双侧颈总动脉 10min组 ,小鼠于术中或术后短时间内死亡。结扎双侧颈总动脉 4、7min存活 4 8h海马CA1区神经元Caspase 3免疫染色阳性 ,且 7min组阳性神经元数明显多于 4min组 ,差异有显著性 (P <0 0 1)。对照组未见阳性神经元。结论　昆明小鼠Willis环不完善 ,双侧颈总动脉结扎能引起严重的大脑皮质和海马缺血 ,这为脑缺血的研究提供了一种新的动物模型。Objective To investigate if there is Willis cycle in the Kunming mouse. Methods Anilin blue was irrigated into the ascending aorta following the legation of bilateral common carotid arteries(BCCA),and the dying of brain were observed. Other mice suffered from the legation of BCCA for 4 min?7 min or 10 min and 48 h reperfusion. It was investigated the expression of Caspase 3 in hippocampus CA1 region by immunohistochemical method. Results To the mice with BCCA ligation,no dying was observed in bihemisphere and hippocampus,but dying was observed in cerebrum and brain stem. To control mice,the whole brain was dyed. All mice with 10 min BCCA ligation died on operation or short time after operation. The expression of Caspase 3 was increased in the neurons of CA1 region of hippocampus with 4 min?7 min BCCA ligation and 48 h reperfusion,moreover,the increase in 7 min group was significantly more(P<0 01). Conclusion The Willis cycle of Kunming mouse isnt complete;the ligation of BCCA can lead to severe ischemia of cerebral cortex and hippocampus. Kunming mouse is a new animal model for cerebral ischemia.

关 键 词：昆明小鼠 脑缺血 再灌 CASPASE-3 大脑动脉环 

分 类 号：R741.02[医药卫生—神经病学与精神病学]