应用生物素标记探针对癌周肝硬变组织中HBV-DNA的原位分子杂交研究  

IDENTIFICATION OF HBV-DNA IN CIRRHOTIC LIVER TISSUE BY IN SITU HYBRIDIZATION WITH BIOTIN-LABELED PROBES

作　　者：丛文铭[1] 吴孟超[1] 张秀忠[1] 屠振兴[1] 

机构地区：[1]第二军医大学肝胆外科研究所

出　　处：《肿瘤》1991年第4期152-153,共2页Tumor

摘　　要：在肝癌病因学的研究中,HBV感染与肝癌发生的关系最受到重视。许多资料显示,在乙肝及肝癌组织内有HBV-DNA的存在。然而,以往的此类研究多以Southern吸印法为主,难以获得HBV-DNA在单个肝细胞内的定位证据。本文应用晚近发展起来的原位分子杂交技术,对26例肝硬变组织中单个肝细胞内HBV-DNA的存在状态和分布特征进行了定位研究,讨论了可能的生物学意义,现报告如下。The biotin-labeled RBV-DNA probes were used to localize RBV-DNA in singlehe patocytes of 26 cases of liver cirrhosis by in situ hybridization. The results showed thatHBV-DNA hybrid grains were detected in 14 cases (53. 9%), in which 9 cases showedpositive hybridization in hepatocytoplasms, and 5 cases showed positive grains meinlyin hepatocytoplasms but also in hepatocytic nuclei. It suggests that HBV-DNA in mosthepatocytes in liver cirrhosis was in a free state. So, it could have clinical significans.to prevent the developmeot and postoperative recurrence of hepatocellular carcinoma byinhibiting and eliminating HBV.

关 键 词：肝肿瘤 癌周组织 生物素 标记探针 HBV 

分 类 号：R735.702[医药卫生—肿瘤]