胰腺癌组织中纤溶成份的表达及其意义  

Activation of the serine proteinase :positive association with pancreatic cancer progression

作　　者：汤文浩[1] 李国强[1] 王凤臣[1] 高乃荣[1] 嵇振岭[1] 杨德同[1] 王尔慧[1] 

机构地区：[1]南京东南大学附属中大医院普外科,江苏210009

出　　处：《中国肿瘤临床与康复》2002年第5期11-13,共3页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：本课题受国家教委留学归国人员科研启动基金 (编号 :98LX1);南京市人事局非教育系统留学回国人员科技活动基金资助 (编号 :98LX3 )

摘　　要：目的　研究肿瘤组织中纤溶成份的表达与肿瘤进展和术后生存时间的关系。方法　用Northern印迹和酶联免疫吸附法研究了 10例正常胰腺组织标本和 3 0例胰腺癌切除标本的尿激酶型纤溶酶原激活物 (uPA)、尿激酶型纤溶酶原激活物受体 (uPAR)和纤溶酶激活抑制物 1(PAI 1)表达。结果　Northern印迹表明胰腺癌组织与正常胰腺组织相比uPA、uPAR和PAI 1mRNA的表达分别升高 5 .2倍 ,3 .5倍和 9.7倍 (P <0 .0 1)。 77% (2 3 / 3 0 )的肿瘤患者表现为uPA、uPAR和PAI 1同步升高。免疫吸附分析显示肿瘤组织中uPA活性比正常组高 1.7倍 (P <0 .0 1)。uPA、uPAR和PAI 1同步表达者术后生存时间 (中位 9个月 )明显低于非同步高表达组 (中位 18个月 ) (P <0 .0 1)。结论　本研究表明 ,uPA、uPAR和PAI 1在胰腺癌组织中广泛存在 ,并且往往表现为同步高表达 ;uPA、uPAR和PAI 1同步高表达者术后生存时间短。Objective uPA(urokinase typeplasminogenactivator) ,uPAR(urokinase typeplasminogenactivatorrecep tor)andPAI 1 ( plasminogenactivatorinhibitortype 1 )belongtotheplasminogenactivationsystem .Upregulationofthesefac torshasbeenreportedinseveralmalignanciesandacorrelationwithshorterpostoperativesurvivalcouldbeestablishedinbreast cancerandkidneycancer.InthepresentstudyweexaminedtheexpressionofthethreefibrinolyticcomponentsuPA ,uPARand PAI 1andtheirrelationtothebiologicalgrowthbehavioranditsinfluenceonsurvivalinpancreaticcancer.Methods Pancreatic cancertissueswereobtainedfrom 30 patients( 1 8men ,1 2women ;medianage 6 6 years)undergoingpancreaticresction .1 0nor malpancreatictissuesampleswereobtainedfrompreviouslyhealthyindividualsduringmultiorgandonationfortransplantation . mRNAexpressionofuPA ,uPARandPAI 1wasanalyzedbyNorthernblotanalysisanduPAactivitywasinvestigatedbyim munosorbentassay .Inadition ,NorthernblotanalysisofuPA ,uPARandPAI 1andimmunosorbentanalysisofuPAactivitywas correlatedwithhistopathologicalandclinicalpatientdata .Results Northernblotanalysisrevealeda 5.2 fold ,a 3.5 foldanda 9.7 foldincreaseinuPA ,uPAR ,andPAI 1mRNAlevelsinpancreaticcancer ,respectively ,incomparisonwithnormalcontrols (P <0 .0 1 ) .ThemRNAmoietiesofuPA ,uPARandPAI 1wereconcomitantlyincreasedin 77%ofthepatients .Immunosor bentanalysisdemonstratedthatuPAactivitywas 1 .7 foldincreasedinpancreaticcancercomparedwithnormalcontrols(P <0 . 0 1 ) .CorrelationofNorthernblotdatawiththeclinicalparametersofthepatientsindicatedthatpatientswithconcomitantover expressionofuPA ,uPARandPAI 1hadashorterpost operativesurvival(median 9months)thanpatientswithonlyoneortwo overexpressionofthesefactors(median 1 8months) (P <0 .0 1 ) .Conclusion OurdatarevealthatoverexpressionofuPA ,uPAR andPAI 1isoftenpresentinhumanpancreaticcarcinomas .Concomitantupregulationofthesefactorsmaycreateanenviron mentthatenablespancreaticcancercellstoinvadesurroundingtissuesandserveasprognosticmarkersinhumanpancreaticcan cer . [

关 键 词：胰腺癌 纤溶成份 预后 NORTHERN杂交 酶联免疫吸附法 UPA UPAR PAI-1 

分 类 号：R735.9[医药卫生—肿瘤]