玉米弯孢菌叶斑病抗性的QTL分析  

QTL Analysis of Resistance to Curvularia lunata in Maize

作　　者：黎裕[1] 戴法超[1] 景蕊莲[1] 王天宇[1] 杜金友[1] 贾继增[1] 

机构地区：[1]中国农业科学院作物品种资源研究所,北京100081

出　　处：《中国农业科学》2002年第10期1221-1227,共7页Scientia Agricultura Sinica

基　　金：国家青年"863"资助项目

摘　　要：通过利用AFLP和SSR标记 ,对丹 340×沈 135的F2∶3 群体 (113个家系 )进行玉米弯孢菌叶斑病抗性基因的遗传作图和QTL分析 ,得到如下结论 :(1)玉米弯孢菌叶斑病抗性是由多基因控制的 ;(2 )应用复合区间作图法 ,对 1999年的玉米全株抗病性 ,检测到 4个QTL ,分别位于第 6、6、8和 10染色体上 ,可解释表型变异的 4 9.9% ;对 2 0 0 0年的玉米全株抗病性 ,检测到 6个QTL ,2个位于第 6染色体上 ,3个位于第 7染色体上 ,1个位于第 10染色体上 ,可解释表型变异的 77.6 % ;第 10染色体上的QTL是 2年间共同的QTL ,来自抗病亲本沈 135 ;(3)对每个QTL(定量特征点位分析 ) ,均检测到加性和显性效应 ,但相对大小有不同 ,各QTL以部分显性、显性和超显性为主要遗传方式 ;(4)控制玉米弯孢菌叶斑病抗性的QTL之间存在上位性互作。Molecular characterization and quantitative trait loci (QTL) analysis on the resistance to Curvularia lunata were carried out in this study, using AFLP and SSR markers and an F 2∶3 population of 'Dan 340 (susceptible parent) ×Shen 135 (resistant parent)'. The major results and conclusions are described as follows: (1) The resistance to Curvularia lunata in maize and the agronomic traits studied were controlled by multiple genes. (2) Through composite interval mapping, four QTLs were detected for resistance to Curvularia lunata phenotyped in 1999, which were located on chromosome 6, 6, 8 and 10, respectively and totally accounted for 49.9% of phenotypic variation; six QTLs were detected for resistance to Curvularia lunata phenotyped in 2000, two of which were located on chromosome 6, three on chromosome 7 and one on chromosome 10, and totally accounted for 77.6% of phenotypic variation. The quantitative trait locus on chromosome 10 which was derived from Shen 135 (resistant parent) was common in the two years. (3) For each QTL, both additive effect and dominant effect were detected. Most QTLs had the genetic effect of partial dominance, dominance or overdominance. (4) Digenic epistasis interaction existed between some QTLs controlling resistance to Curvularia lunata.

关 键 词：玉米 弯孢菌叶斑病 抗病性 QTL 分子标记 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]