全反式维甲酸对神经母细胞瘤细胞系体外生长的影响  

The effect of all trans retinoic acid on the growth of neuroblastoma cell line in vitro

作　　者：王希华[1] 陈万涛[2] 岳孟源[1] 何荣根[2] 步荣[1] 

机构地区：[1]上海第二医科大学附属新华医院上海儿童医学中心,200127 [2]上海第二医科大学附属第九人民医院

出　　处：《肿瘤》2002年第5期394-396,共3页Tumor

基　　金：国家自然科学基金部分资助 (编号 :3 9670 787)

摘　　要：目的　体外观察全反式维甲酸 (alltransretinoicacid ,ATRA)对神经母细胞瘤细胞系 (SK N SH)细胞生长的影响。方法　SK N SH细胞持续培养在含ATRA的 96孔板中 ,采用MTT法检测ATRA的体外抗增殖效果 ,用相差倒置显微镜及透射电镜观察细胞形态变化。结果　 0 .1～ 1μmol/L浓度的ATRA对SK N SH细胞可产生明显的抗增殖作用 ;其抗增殖作用呈时间和剂量依赖关系。 1μmol/LATRA作用 12天 ,细胞生长抑制率达 77.0 3%。ATRA能诱导SK N SH细胞分化成熟 ,先是细胞出现神经树 (轴 )突样胞突 ,培养 10天后多个细胞形成类似神经节样形态结构 ,“神经节”之间形成类神经纤维 ;电镜观察细胞核浆比例下降 ,无特征性细胞凋亡形态变化。结论　ATRA通过诱导分化作用 ,而明显抑制神经母细胞瘤细胞增殖。Objective To investigate the effect of all trans retinoic acid on antiproliferation of cells of SK-N-SH cell line. Methods SK-N-SH cells were incubated in the continued presence of ATRA in 96 well plate. MTT assay was used to detect the efficiency of inhibiting growth in SK-N-SH cell. Morphologic changes were observed using phase-contrast microscopy. Results ATRA at the concentration of 0.1～1μmol/L had the antiproliferative effect on SK-N-SH cells and showed time and dose dependent manner. The inhibiting rate reached 77.03% while SK-N-SH cells exposured to ATRA at the concentration of 1μmol/L for 12 days. ATRA can induce neuroblastoma cells to extend neurite-like processes. After day 10, the cultures resembled the differentiated neural crest cell, then the cells continued to round up and detach from the plate. Conclusion ATRA can suppress neuroblastoma cells growth by inducing cell differentiation.

关 键 词：MTT法 全反式维甲酸 神经母细胞瘤 体外生长 影响 

分 类 号：R730.264[医药卫生—肿瘤]