BEP2D细胞和R15Hp35T-2细胞中60个肺癌相关基因的表达研究  

Expression profile of 60 lung cancer related genes in BEP2D cell and R15Hp35T-2 cell

作　　者：范保星[1] 孙敬芬[1] 张开泰[1] 吴德昌[1] 

机构地区：[1]军事医学科学院放射医学研究所分子毒理研究室,北京100850

出　　处：《中国肺癌杂志》2002年第5期321-325,共5页Chinese Journal of Lung Cancer

摘　　要：目的　研究永生化人支气管上皮细胞 (BEP2D)和α粒子辐射诱发BEP2D细胞后形成的恶性转化细胞 (R15Hp35T 2 )中 6 0个肺癌相关基因的表达谱。 方法　首先搜集了 6 0个肺癌相关的基因 ,经扩增和纯化后 ,用CartesianPixSys5 5 0 0cDNAMicroarray点样仪将 6 0个肺癌相关基因以微阵列形式点布于醛基化的玻璃片上。然后提取BEP2D细胞和R15Hp35T 2细胞的RNA ,经长片段反转录和线性扩增标记成荧光探针后与微阵列中的cDNA进行杂交。结果　与BEP2D细胞相比 ,R15Hp35T 2细胞中上调表达的基因有 2 7个 ,下调表达的基因有 7个。大部分抑癌基因的mRNA丰度在 2种细胞中表达相似 ;而大多数癌基因和生长因子类基因的mRNA丰度在R15Hp35T 2细胞中高表达。结论　在辐射诱发的人支气管上皮恶性转化细胞中 。Objective To profile the expression patterns of 60 lung cancer related genes in human bronchial epithelial cell (BEP2D) and alpha-particle induced malignantly transformed cell (R15Hp35T-2). Methods Sixty lung cancer related cDNAs were micro-arrayed onto the microscope slides using Cartesian PixSys5500 cDNA Microarray machine. Total RNA from BEP2D cell and R15Hp35T-2 cell was extracted and labeled by fluorescent dye. The labeled probe was then hybridized with the cDNA. Results Compared with the BEP2D cell, 27 genes up-regulated and 7 down-regulated in the R15Hp35T-2 cell. The expression abundance of most tumor suppressor genes were similar in the two kinds of cells, however, most oncogenes and growth factor genes were overexpressed in R15Hp35T-2 cell. Conclusion In malignantly transformed human bronchial epithelial cell model induced by alpha-particle, some oncogenes and growth factor genes may promote the malignant transformation together.

关 键 词：CDNA MICROARRAY BEP2D细胞 基因表达谱 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]