地高辛素标记探针原位杂交法检测B淋巴瘤CD23基因的表达  

Detection of CD23 mRNA expression in B-cell lymphoma by in situ hybridization with digoxigenin-labelled DNA probe

作　　者：王琳[1] 吴斌[2] 苏春晓[1] 李树浓[3] 

机构地区：[1]海口市人民医院血液科,海南海口570208 [2]海南省人民医院血液科,海南海口570311 [3]中山医科大学病理生理学教研室,广东广州510089

出　　处：《中国病理生理杂志》2002年第9期1074-1076,共3页Chinese Journal of Pathophysiology

基　　金：海南省自然科学基金资助项目 (No .396 0 9)

摘　　要：目的 :研究B淋巴瘤CD2 3基因表达。方法 :SP免疫组化选出 2 0例B淋巴瘤并进行CD2 3基因蛋白(膜CD2 3)检测 ,地高辛素标记CD2 3DNA探针原位杂交检测CD2 3mRNA。结果 :膜CD2 3的阳性率 90 % ,CD2 3mRNA的阳性率 90 %。结论 :CD2 3在B淋巴瘤中高表达 ,这为研究CD2 3在B淋巴瘤中的发病机制和临床诊治提供了重要资料。AIM: To detect CD23 mRNA expression in B-cell lymphoma. METHODS: 20 pathological diagnostic conformed B-cell lymphoma samples were selected. Surface CD23 protein expression was examined by SP-immunohistochemistry and CD23 mRNA was detected by in situ hybridization with digotoxin labeled CD23 DNA probe.RESULTS: The positive rates of both surface CD23 protein and CD23 mRNA expression in B-cell lymphoma samples were more than 90%. CONCLUSIONS: The high expression of CD23 was showed in B-cell lymphoma both in mRNA and in protein levels. The results of this study was useful for understanding the molecular mechanism of B-cell lymphoma and for clinical diagnosis and treatment of the disease.

关 键 词：原位杂交 地高辛配基 DNA探针 B细胞淋巴瘤 IGE受体 

分 类 号：R733.402[医药卫生—肿瘤]