树突状细胞对肿瘤浸润性淋巴细胞杀伤自体肝癌细胞活性影响的研究  

Study of killing activity of tumor infiltrating lymphocytes on autogenous hepatocarcinoma cells enhanced by dendritic cells

作　　者：刘剑勇[1] 张力图[2] 李挺[1] 赵荫农[1] 张春燕[2] 陈建思[1] 覃宇周[1] 吴飞翔[1] 唐凯[2] 

机构地区：[1]广西医科大学附属肿瘤医院肝胆外科,广西南宁530021 [2]广西医科大学附属肿瘤医院细胞与分子生物学研究室,广西南宁530021

出　　处：《实用肿瘤杂志》2002年第5期311-313,共3页Journal of Practical Oncology

基　　金：广西科学研究与技术开发计划攻关项目 (桂科攻 0 14 30 5 2 )

摘　　要：目的　探讨树突状细胞 (DC)激活的肿瘤浸润性淋巴细胞 (TIL )体外对自体肝癌细胞杀伤活性。方法　从肝癌患者外周血获取 DC,应用粒 /巨噬细胞集落刺激因子 (GM- CSF)、白细胞介素 - 4 (IL - 4 )和肿瘤抗原激活 DC,然后用 DC激活 TIL ,观察 TIL在体外对自体肝癌细胞的杀伤活性。结果　 DC激活的 TIL对自体肝癌细胞具有很高的杀伤活性 ,杀伤率为 (89.39± 3.0 5 ) % ,明显高于未经 DC激活的 TIL、DC激活的 T淋巴细胞和未经 DC激活的 T淋巴细胞对自体肝癌细胞的杀伤率。其杀伤率分别为 (5 5 .2 3± 1.5 3) %、(5 4 .89± 1.4 8) %和 (3.6 5± 0 .2 6 ) %。结论　肝癌患者外周血 DC能诱导Objective To study the killing activity of tumor infiltrating lymphocytes (TIL) stimulated by dendritic cells (DC) on autogenous hepatocarcinoma cells in vitro. Methods DCs were isolated from peripheral blood of hepatocarcinoma patients. DCs were stimulated by granulocyte/macrophage colony stimulating factor (GM CSF),interleukin 4 (IL 4) and tumor antigen. Then TIL was incubated with activated DCs and their killing activity on autogenous hepatocarcinoma cells in vitro were observed. Results TIL stimulated by DCs presented high killing activity on autogenous hepatocarcinoma cells and the killing rate was (89.39±3.05)%. The killing rate was markedly higher than that of TIL not stimulated by DC and T lymphocytes stimulated by DC or not on autogenous hepatocarcinoma cells, respectively (killing rate: (55.23±1.53)%, (54.89± 1.48)% and (3.65±0.26)%, respectively).Conclusion The results indicate that DC from patients with hepatocarcinoma can induce efficient and specific anti hepatocarcinoma immune response.

关 键 词：树突状细胞 肿瘤浸润性淋巴细胞 肝肿瘤 免疫活性 

分 类 号：R735.7[医药卫生—肿瘤]