一氧化氮对血管内皮细胞凋亡的影响  

The Study of the Effect of Nitric Oxide on Apoptotic Vascular Endothelial Cells

作　　者：王林林[1] 魏文宁[1] 陈智超[1] 胡豫[1] 宋善俊[1] 

机构地区：[1]华中科技大学同济医学院血液病研究所,武汉430022

出　　处：《血栓与止血学》2002年第4期162-164,共3页Chinese Journal of Thrombosis and Hemostasis

摘　　要：目的:研究一氧化氮(NO)对人血管内皮细胞凋亡的影响。方法:TNF-α刺激培养的人脐静脉内皮细胞(HUVEC)后,用流氏细胞术Annxin Ⅴ方法检测细胞凋亡情况,用分光光度法检测细胞上清液中NO水平。结果:低浓度NO能抑制TNF-α诱导的内皮细胞凋亡,而高浓度NO能促进内皮细胞凋亡。结论:低浓度NO对HUVEC有抗凋亡作用而高浓度NO则有促凋亡作用。Objective: To explore the effect of nitric oxide on apoptotic vascular endothelial cells. Methods: After stimulating HUVEC with TNF-α, apoptosis was detected by flow cytometry, the NO level in the supernatant was measured by spectrophotography. Results: Low concentration of NO could inhibited the endothelial cells apoptosis induced by TNF-α while high concentration of NO could accelerate apoptosis. Conclusion: The result demonstrates both an anti-apoptosis effect of low concentration of NO and a pro-apoptotic effect of high concentration of NO in the endothelial cells.

关 键 词：一氧化氮 血管内皮细胞 细胞凋亡 TNF-Α 动脉粥样硬化 作用机制 

分 类 号：R364.3[医药卫生—病理学]