维甲酸抑制鼻咽癌端粒酶和hTR表达及诱导凋亡的研究  

Down-regulation of telomerase and its RNA and apoptosis in the HNE_1 cell line of nasopharyngeal carcinoma induced by retinoid

作　　者：文忠[1] 郭梦和[1] 肖健云[2] 田勇泉[2] 赵素萍[2] 唐发清[2] 

机构地区：[1]第一军医大学珠江医院耳鼻咽喉科,广州510282 [2]中南大学湘雅医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉科杂志》2002年第10期539-541,共3页Journal of Clinical Otorhinolaryngology

摘　　要：目的:探讨维甲酸(RA)诱导鼻咽癌端粒酶及hTR表达下降和凋亡改变。方法:10-5mol/L维甲酸处理HNE1细胞后,采用TRAP PCR ELISA、逆转录巢式PCR、凋亡指数及透射电镜检测端粒酶、hTR表达及凋亡改变。结果:加入维甲酸6 d后,HN E1细胞形态不规则,细胞悬浮,细胞数减少,端粒酶及hTR表达受抑制,间接定量显示端粒酶活性(0.68±0.14)U只及对照组(1.16±0.46)U的1/2,凋亡指数及细胞明显高于对照组(P<0.05)。结论:维甲酸能明显抑制鼻咽癌细胞中端粒酶及hTR表达,并诱导该类肿瘤细胞增殖受阻及发生凋亡改变。Objective:To explore the down-regulation of telomerase as well as its RNA and apoptosis induced by retinoid acid. Method: After HNE1 cell lines of NPC were incubated with 10-5 mol of retinoid in 6 days, the expression of telomerase and its RNA and the apoptosis of HNE1 cell lines were detected respectively with TRAP PCR ELISA, RT-nested PCR, apoptosis indexes and trans-electron microscope. Result; After 6 days when RA was added to the HNE1 cell line, the morphological change and suspending of HNE1 cells were found and the HNE1 cells reduced. Meanwhile, the expression of telomerase and hTR was obviously inhibited and the telomerase activity was only the 1/2 of the control with the use of ELISA indirect quantitative assay. Apoptosis indexes and apoptosis cells in RA groups were higher than those in control ( P <0. 05). Conclusion: The expression of telomerase and hTR are obviously inhibited and the apoptosis of HNE1 cell lines is also induced by retinoid.

关 键 词：鼻咽肿瘤 维甲酸 端粒酶 端粒酶亚单位 细胞凋亡 

分 类 号：R739.63[医药卫生—肿瘤]