全脑缺血再灌注对大鼠延髓内脏带NADPH-d阳性神经元分布与Fos蛋白表达的影响  

EFFECTS ON DISTRIBUTION OF NADPH-d POSITIVE NEURONS AND Fos EXPRESSION IN THE MEDULLARY VISCERAL ZONE OF THE RAT FALLOWING CEREBRAL ISCHEMIA REPERFUSSION INJURY

作　　者：晋光荣[1] 杨劲松[1] 杨宁[1] 

机构地区：[1]东南大学基础医学院解剖学教研室,南京210009

出　　处：《解剖学报》2002年第5期479-483,共5页Acta Anatomica Sinica

摘　　要：目的　观察大鼠全脑缺血 再灌注不同时间点延髓内脏带 (MVZ)内NADPH d阳性神经元与Fos蛋白表达的变化及相互关系。　方法　采用 4血管闭塞 (4VO)改良法以及运用NADPH d组织化学反应和Fos免疫组织化学反应及双重染色技术对全脑缺血 30min ,再灌注 2h、4h、8h、12h、2 4h、4 8h对MVZ进行研究。　结果　脑缺血组MVZ内NADPH d阳性神经元染色反应增强和Fos蛋白表达随脑缺血后再灌注时程而变化 ,并见有 15 %～ 2 0 %阳性双染细胞。　结论　在全脑缺血再灌注过程中 ,MVZ内NADPH d阳性神经元与Fos蛋白表达的分布变化具有相关性 。Objective To investigate the changes of distribution and expression of NADPH\|d positive neurons and Fos like immunoreactive(Fos\|LI) neurons in the rats medullary visceral zone(MVZ) following different time course after global cerebral ischemia reprefussion. Method Fifty adult SD rats were divided into three groups,normal control group( n =2),sham operation group( n =12) and ischemia group( n =36).The 4 brain vessel of ischemia group rats were occluded for 30?min,then reperfused for 2?h,4?h,8?h,12?h,24?h and 48?h respectively.The sections of medulla oblongata were divided into three series.The first and second series were reacted with NADPH\|d histochemical and anti\|Fos immunohistochemical methods respectively,and the third series were double stained with the other two methods mentioned above. Results NADPH\|d neurons and Fos\|LI neurons were distributed in bilateral MVZ(in NTS and VLM predominately).The number of NADPH\|d neurons and Fos\|LI neurons of ischemia group rats were significantly increased than that of control group at 2\|12?h.Approximately 15\|20 percent NADPH\|d/Fos\|LI double labeled neurons were found in MVZ.Conclusion\ The results indicated that NADPH\|d neurons and non\|NADPH\|d neurons could sensitively respond to brain ischemia reperfsion and might associate with regulation of stress and defence responese.

关 键 词：缺血再灌注 大鼠 管髓内脏带 NADPH-D 阳性神经元 FOS蛋白 全脑缺血 免疫组织化学 

分 类 号：R743.31[医药卫生—神经病学与精神病学]