扇贝多肽保护中波紫外线损伤HeLa上皮细胞的研究  

The Protective Effects of Polypeptides from Chlamys farreri against UVB Radiation

作　　者：于业军[1] 刘晓萍[2] 王春波[2] 

机构地区：[1]青岛大学医学院附属医院皮肤科 [2]青岛大学医学院

出　　处：《中华皮肤科杂志》2002年第5期374-377,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金资助课题(39970638)

摘　　要：目的探讨扇贝多肽对中波紫外线(UVB)照射下HeLa上皮细胞氧化损伤的保护作用。方法建立UVB(辐照强度为7.15×10-5J/cm2)对HeLa上皮细胞氧化损伤模型。HeLa上皮细胞随机分为6组,未辐射对照组、辐射损伤组犤损伤对照组、0.5%扇贝多肽(PCF)组、1%PCF组、2%PCF组、1%维生素C组犦。四唑盐比色法测定细胞活性;流式细胞仪测定细胞的凋亡率、死亡率和胞内游离Ca的含量;酶法测定抗氧化酶(谷胱甘肽、超氧化物歧化酶、过氧化氢酶、丙二醛)的含量及总抗氧化能力。结果离体条件下扇贝多肽:①显著增强HeLa上皮细胞的活性;②降低HeLa上皮细胞的凋亡率和死亡率;③显著提高HeLa上皮细胞内游离Ca的含量;④提高细胞上清液中谷胱甘肽、超氧化物歧化酶、过氧化氢酶的活性,降低丙二醛含量,且呈量效正比关系。结论扇贝多肽在体外有抗UVB氧化损伤的作用。其作用机制可能是扇贝多肽通过提高抗氧化酶含量、清除自由基等,以减轻细胞凋亡而发挥其保护作用。Objective To study the protective effects of th e polypeptides isolated from Chlamys farreri(PCF)against ultraviolet B radiatins.Methods An oxidative damage model of HeLa epi thelial cell line irradiated by ultraviolet B(UVB 7.15×10 -5 J /cm 2 )was established.The HeLa cells were randomly divided into six groups:control group,UVB radiated groups(radiated control,0.5%PCF,1%PCF,2%PCF,1%Vit C).The enzymic activities of HeLa cells were tested by MTT methods,the apoptosis rate an d death rate of HeLa epithelial cells and the content of free calcium in those cells were tested by using flow cytometry(FCM).The contents of MDA and the activities of GSH-Px ,SOD,CAT in the supernatants of cells were determin ed by biochemical methods.Results The results indicated that polypept ides fromChlamys farreri could enhance the activities of GSH-Px,SOD,CA T and decrease the contents of MDAin the su pernatants of HeLa cells,increase t he concentration of cellular free calciumin the cells,and decrease the rates of apoptosis and cell death of the cells .Conclusions The PCF has the protective effects on the HeLa cells irradiated by ultraviolet B.The mechanism of th is effects of PCF may be mediated by e-liminating the free radicals,by increasing the activities of antioxidative enzymes to inhibit the oxidativ e damages caused by UVB .

关 键 词：扇贝多肽 紫外线 HELA细胞 抗氧化药 辐射损伤 皮肤损伤 

分 类 号：R758.1[医药卫生—皮肤病学与性病学]