抑癌基因p16在成釉细胞瘤及牙源性角化囊肿中表达的研究  

An Investigation of p16 Expression in Ameloblastoma and Odontogenic Keratocyst

作　　者：钟鸣[1] 王洁[1] 王兆元[1] 李瑞武[2] 

机构地区：[1]中国医科大学口腔医学院,沈阳110002 [2]中国医科大学附属第一临床学院口腔颌面外科,沈阳110002

出　　处：《口腔医学》2002年第3期115-117,共3页Stomatology

基　　金：辽宁省教育厅资助课题(编号:20122171)

摘　　要：目的 研究成釉细胞瘤（Ameloblastoma,AB）中 p16蛋白及 p16 mRNA表达,探讨其生物学意义。方法 应用免疫组化方法（SP）检测40例 AB及牙源性角化囊肿（Odontogenic keratocyst,OKC）7例,基底细胞癌（Basal cell carcinoma,BCC）8例 p16蛋白的表达,同时用原位杂交法检测 p16 mRNA在20例 AB中表达。结果 40例AB,7例 OKC,8例 BCC,p16蛋白缺失表达分别为70％,85.7％,100％。p16mRNA缺失率80.5％,与蛋白缺失表达一致率53.8％。结论 （1）AB与 OKC的发生与p16蛋白缺失有关。（2）AB中原发与复发比,原发与恶变比 p16蛋白缺失率不同（P<0.05）。（3）AB及 OKC,BCC三种不同病变经统计学处理 P>0.05,末见明显相关性。（4）在 AB中p16蛋白与 p16 mRNA表达 P<0.05,具有一定相关性。Objective To study p16 protein and mRNA expression in ameloblastoma (AB)and to investigate biological characteristics of AB.Methods The immunoactivity of p16 protein in 40 cases of AB,7 cases of odontogenic keratocyst(OKC) ,8 cases of basal cell carcinoma (BCC) was determined by Streptavidin-Biotin method. p16 mRNA of 20 cases of AB was detected in situ hybrization. Results The ratio of expression lost of p16 protein in AB, OKC, BCC is 70% ,85.5% , 100% , respectively. The ratio of expression lost of p16 mRNA in AB is 80.5 % , and the consistency ratio with p16 protein is 53.8 % . Conclusion 1. The development of AB and OKC is related with expression lost of p16 protein.2. Comparing recurrent and malignant AB with primary AB,there is a great difference in the expression level of p16 protein( P < 0.05) .There is no difference in p16 expression in AB,OKC,BCC(P > 0.05) .3. The expression of p16 protein and mRNA in AB is related to each other (P<0.05).

关 键 词：牙源性角化囊肿 牙源性肿瘤 成釉细胞瘤 P16蛋白 p16mRNA 免疫组织化学 AB OKC 基因表达 

分 类 号：R739.82[医药卫生—肿瘤]