新型磷酸酯表面活性剂的合成、表征及其反胶束体系对蛋白质的提取  

Synthesis and Characterization of Double-tailed Phosphate Surfactant and Extraction of Protein with Its Reversed Micelles

作　　者：阎云[1] 褚莹[1] 林毅[1] 邢双喜[1] 刘莹莹[1] 刘景林[1] 吴子生[1] 

机构地区：[1]东北师范大学化学学院,长春130024

出　　处：《高等学校化学学报》2002年第10期1921-1925,共5页Chemical Journal of Chinese Universities

基　　金：吉林省科学技术委员会应用基础项目 (批准号 :990 5 5 1)资助

摘　　要：合成了适于反胶束法提取蛋白质的磷酸酯表面活性剂二油酸乙二醇单酯基磷酸酯钠 (DEOPA) ;以氢核磁共振谱和红外光谱表征了其结构 ;测定了其临界胶束浓度 (cmc)、亲水亲油平衡值 (HLB值 )及对水的增溶作用等表面化学性质 ;考察了 DEOPA/异辛烷反胶束体系提取蛋白质的性能 .结果表明 ,DEOPA具有较高的表面活性 ,适合于构建反胶束体系 。A new phosphate surfactant that has never been reported before, sodium di-ethyloleyl phosphate acid(DEOPA), which fits for the extraction of proteins with reversed micellar system, has been synthesized. Its structure has been characterized with 1H NMR and FTIR spectrum. Its surfacial physico-chemical properties, such as cmc(Critical Micellar Concentration), HLB(Hydrophilic-Lipophilic Balance), and its solubilization effect to water was detected. Extraction of proteins using reverse micelles composed of DEOPA and isooctane was carried out. The results show that DEOPA is of relatively high surfactant activity, which means it fits for composing reversed micellar systems, and is of superiority when extracting large molecular weight proteins.

关 键 词：表征 双链磷酸酯 表面活性剂 合成 反胶束 提取 蛋白质 分离 纯化 DEOPA 异辛烷 

分 类 号：Q51[生物学—生物化学] Q503