不同解冻方法对奶牛冻精活力的影响  

Effects of different thawing methods on post-thawed motility of cryopreserved cow semen

作　　者：卜书海[1] 孙志杰[2] 李青旺[2] 于永生[2] 王立强[2] 胡建宏[2] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100 [2]西北农林科技大学动物科技学院,陕西杨陵712100

出　　处：《西北农林科技大学学报（自然科学版）》2002年第5期40-43,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省科技攻关项目 (4 13 0 3 0 3 )

摘　　要：将 5头荷斯坦种公牛细管冻精在不同温度、不同时间下进行解冻 ,对解冻后的精液品质进行检测。结果表明 :解冻后顶体正常率、精子活率随解冻温度升高而升高 ,而畸形率随解冻温度升高而降低 (P<0 .0 1) ;高温(37℃ ,75℃ )下随着解冻时间延长 ,活率增高 (P<0 .0 5 ) ;低温 (5℃ )、室温 (15℃ )下解冻 ,活率与解冻时间长短没有明显关系 (P>0 .0 5 ) ;精子顶体正常率、畸形率、存活时间与解冻时间无显著关系。在实践中建议使用 75℃ 2～6 s解冻。Sixty frozen bovine semen straws (0.25 mL) collected from five Holstein breeding bulls are thawed to determined the effects of different thawing temperature and times on properties of bull semen.The results show that:(1) With the increasing of thawing temperature,the proportion of motile spermatozoa,the proportion of acrosomal integrity,the motible length get improved significantly( P <0.05).Abnormal spermatozoa rate decreased significantly with the increasing of thawing temperature ( P <0.05).(2) The percent of progressive spermatozoa thawed in 37 ℃ or 75 ℃ is higher with the increasing of thawing time,but the percent of progressive spermatozoa is no relation to thawing time.(3) There is no relation among thawing time and the proportion of motile spormatozoa,the proportion of acrosomal integrity,the motible length.It is suggested to thaw at 75 ℃ for 2~6 seconds in practice.

关 键 词：解冻方法 奶牛 冻精活力 解冻温度 解冻时间 精液品质 

分 类 号：S823.91[农业科学—畜牧学]