肝癌细胞形态和生长行为对基底拉伸的响应  

Effect of Mechanical/Stretch on Growth and Morphology of Hepatocellular Carcinoma Cell

作　　者：王红兵[1] 黄岂平[1] 秦建[1] 卢晓[1] 王远亮[1] 蔡绍皙[1] 

机构地区：[1]重庆大学生物工程学院生物力学与组织工程教育部重点实验室,重庆400044

出　　处：《重庆大学学报（自然科学版）》2002年第9期66-67,74,共3页Journal of Chongqing University

基　　金：国家自然科学基金重点项目 (1973 2 0 0 3 )

摘　　要：采用“四点弯曲梁加载装置” ,对培养的人肝癌细胞株SMMC - 772 1施以拉伸刺激 ,通过显微计算机图像处理系统了解其形态变化 ,流式细胞仪了解其增殖行为 ,结果发现 1)加载 2 4h后SMMC -772 1G2 -M期 8.6 3% ,对照组 15 .92 % ;2 )加载 72h后SMMC - 772 1铺展投影面积缩小 ;3)加载 2 4h后SMMC - 772 1分裂指数 0 .4 6 ,对照组 0 .5 5。加载可部份抑制SMMC - 772In order to research the effect of stretch on the cells, the four-point supporting bending system is applied to stretch the SMMC-7721 hepatocellular carcinoma cells. Then, the proliferation of the cells was determined by FCM, and the morphology is measured by computer-image-processing, the result show that (1) The percent of G 2-M phase of SMMC-7721 is reduced after 24 h stretch (8.63% in 24 h stretched group and 15.92% in control). (2) The project area of SMMC-7721 is reduced after 72 h stretch. (3) The proliferation of SMMC-7721 is inhibited by stretch (the division index of SMMC-7721 in 24 h stretched group is 0.46, in control group is 0.55).

关 键 词：肝癌细胞 形态 生长行为 基底拉伸 SMMC-7721 机械拉伸 生长抑制 

分 类 号：R735.7[医药卫生—肿瘤]