两类移行上皮细胞癌的分子生物学基础  

Molecular biological basis of two classes of transtional cell bladder cancer

作　　者：畅继武[1] 肖向炜[1] 高伟 马腾骧[1] 张淑敏[1] 隋志方[1] 张和顺[1] 张新[1] 王锦[1] 齐湘杰[1] 孙光[2] 马富玲[2] 李振莉[2] 孙明 

机构地区：[1]天津市泌尿外科研究所,300211 [2]天津医科大学附属第二医院 [3]天津市第三中心医院

出　　处：《中华泌尿外科杂志》2002年第10期583-586,共4页Chinese Journal of Urology

摘　　要：目的　探讨两类移行上皮细胞癌中端粒酶活性和脆性组氨酸三联基因 (FHIT)缺失率 ,从而区别两类移行上皮细胞癌的生物学特性。　方法　采用TRAP HSLC定量检测端粒酶活性 ,原位PCR检测FHIT基因缺失率。　结果　两类移行上皮细胞癌端粒酶活性不同 ,Ⅰ类癌第一主峰面积一般 <30 0 0 ,Ⅱ类癌第一主峰面积往往 >30 0 0 ;Ⅰ类癌FHIT基因表达缺失率为 4 / 15 ,Ⅱ类癌FHIT基因表达缺失率为 8/ 11,阳性率差别具有显著性意义 (P >0 .0 5 )。Objective To clarify 2 grades (class) of transitional cell cancer of bladder on molecular biological characters. Methods TRAP HSLC was used for the quantitative assay of telomerase activity in the bladder cancer tissues and PCR in situ for FHIT gene absence rate. Results The first peak of compare area of telomerase activity in the Ⅰ clase cancer was less than 3 000 while in the Ⅱ class cancer it was over 3 000.The absence rate of FHIT was 4/15 in the Ⅰ class cancer tissue.being 8/11 in the Ⅱ class cancer ( P <0.05). Conclusions The biological characteristics has been different between the Ⅰ and the Ⅱ class of transitional cell bladder cancer.

关 键 词：分子生物学基础 移行性细胞癌 端粒酶 基因 

分 类 号：R737.1[医药卫生—肿瘤]