鼠颞下颌关节中神经肽Y阳性纤维的分布及创伤后的密度变化  

Distribution of Neuropeptide Y-like Immunoreactive Fiber in Rat Temporomandibular Joint

作　　者：刘向辉[1] 斯方杰 徐正林[1] 张玲[1] 饶志仁[3] 

机构地区：[1]南京解放军八一医院口腔科,210002 [2]第四军医大学口腔医院 [3]第四军医大学神经生物研究所

出　　处：《华西口腔医学杂志》2002年第5期323-325,329,共4页West China Journal of Stomatology

摘　　要：目的 :观察神经肽Y阳性纤维在鼠颞下颌关节中的整体分布及创伤后的密度变化。方法 :将 6组鼠分别于正常时及撞击后第 1、3、7、14及 2 8天处死 ,完整取出颞下颌关节脱钙脱水 ,恒冷切片 ,ABC法漂染后镜检及半定量分析。结果 :神经肽Y阳性纤维广泛分布于颞下颌关节的软组织中 ,主要位于血管尤其是动脉周围 ;创伤前及创伤后 6组的分布面积为 :16 0 4± 2 7 5 ,95 8± 16 4,88 6± 14 5 ,114 3± 17 0 ,135 0± 2 0 7,15 8 6± 19 5 (单位 :mm2 )。结论 :神经肽Y阳性纤维广泛分布于颞下颌关节软组织的血管周围 ;在创伤后密度呈动态变化 。Objective:This investigation aimed at explore the total distribution of neuropeptide Y-like immunoreactive (NPY-LI) fibers and their changes post-trauma in rat temporomandibular joints (TMJs).Methods: Six groups of rats were killed individually before trauma, 1, 3, 7, 14 and 28 days after trauma. TMJs were extracted totally, and the avidin-biotin-peroxidase complex method and image analysis were employed to detect NPY-LI fibers in frozen sections of TMJs.Results: NPY-LI fibers were distributed extensively in TMJs, except the central disc band and bone, and they were mainly located around blood vessels, especially arteries. The densities of fibers in the six groups were 160.4±27.5, 95.8±16.4, 88.6±14.5, 114.3±17.0, 135.0±20.7, 158.6 ±19.5(unit:mm+2).Conclusion: NPY-LI nerve fibers are distributed extensively in the periphery of blood vessels of TMJs and densities changed dynamically when TMJs were impacted. NPY may play an important role in pathologic change of TMJ by regulating local blood circulation.

关 键 词：纤维密度 动物实验 颞下颌关节 神经肽Y 阳性纤维 创伤 

分 类 号：R782.6[医药卫生—口腔医学]