PTEN基因转染对粘液表皮样癌细胞系M_3SP_2增殖的抑制作用  

Transfection of the Exogenous PTEN-induced Growth Inhibition of the Highly Metastatic Mucoepidermoid Carcinoma Cell Line M_3SP_2 in vitro

作　　者：刘斌[1] 吴军正[1] 孙安[2] 李焰[1] 叶维莉[2] 

机构地区：[1]第四军医大学口腔医学院口腔生物学教研室,710032 [2]武警陕西总队医院

出　　处：《华西口腔医学杂志》2002年第5期361-363,373,共4页West China Journal of Stomatology

基　　金：陕西省自然科学基金 (编号 99SM32 );第四军医大学创新工程 (编号CX99A0 11)资助项目

摘　　要：目的 :观察外源磷酸酶和张力蛋白同源物 (PTEN)抑癌基因对高转移性粘液表皮样癌细胞系M3 SP2 体外生长的影响。方法 :应用脂质体介导方法将野生型PTEN基因导入M3 SP2 细胞 ,通过活细胞观察、细胞生长曲线、分裂指数和克隆形成率了解细胞生长状态。结果 :与亲本细胞比较 ,PTEN基因转染细胞数量减少、排列松散 ,部分细胞发生变性或崩解 ;细胞生长缓慢 ,分裂指数显著减至 16 2‰ (P <0 0 1) ,细胞群体倍增时间明显延长 (31 74h) ,细胞生长抑制率达 5 7 0 5 %～ 71 46 % (P <0 0 0 1) ;细胞克隆形成率为 10 40 %～ 14 93% ,克隆形成抑制率高达 6 5 %～ 72 %(P <0 0 1)。结论 :外源野生型PTEN抑癌基因能显著抑制高转移性粘液表皮样癌细胞系M3 SP2Objective:The purpose of this study was to evaluate the effects of the exogenous phosphatase and tensin homology deleted on chromosome 10 (PTEN) gene on in vitro growth of the highly metastatic mucoepidermoid carcinoma cell line M-3SP-2.Methods: The growth of the exogenous PTEN transfected mucoepidermoid carcinoma cells M-3SP-2-PTEN gene was studied by analyzing cell growth curves, mitosis index and clone formation efficiency and compared with its parental cell line M-3SP-2 and the vector pBabe-puro-transfected cell line M-3SP-2-pBp. Results: The doubling time (h) of M-3SP-2, M-3SP-2-pBp and M-3SP-2-PTEN were 24.50, 24.76 and 31.74; the mitosis index (‰) were 53.0±6.20, 49.0±5.24 and 16.2±3.2; the clone formation efficiency (%) were 37.37, 35.01 and 10.40, respectively. The M-3SP-2-PTEN cells also revealed 57.05%～71.46% inhibition of growth from day 3 to7 and 65%～72% inhibition of clone formation compared with the parental cells.Conclusion: These data provide evidence that the exogenous wild-type PTEN have remarkably inhibitory effects on in vitro proliferation of the highly metastatic mucoepidermoid carcinoma cell line M-3SP-2.

关 键 词：PTEN 抑癌基因 粘液表皮样癌 基因转染 细胞生长 脂质体介导 

分 类 号：R739.8[医药卫生—肿瘤]