HPLC鉴定芥蓝中完形硫代葡萄糖甙  

HPLC identification of intact glucosinolates in Chinese kale

作　　者：何洪巨[1] 宋曙辉[1] 王文琪[1] 武兴德[1] 

机构地区：[1]北京市农林科学院蔬菜研究中心,北京100089

出　　处：《现代仪器》2002年第5期10-12,共3页Modern Instruments

摘　　要：本文对芥蓝中硫代葡萄糖甙组成与含量进行研究。结果表明,HPLC梯度洗脱能很好地分离不同硫甙。二极管阵列(DAD)光谱发现,脂肪类硫代葡萄糖甙在225nm处有一个特征吸收峰,而吲哚类硫甙除在225nm处有吸收峰外,在280nm处还有吸收峰。经鉴定,芥蓝中的硫代葡萄糖甙有7种,主要的硫甙为3-丁稀基GS,约占总量的40％～60％。而4-羟基-3-吲哚基甲基GS和4-甲氧基-3-吲哚基甲基GS的含量最低。4-甲基硫氧丁基GS、3-吲哚基甲基GS和1-甲氧基-3-吲哚基甲基GS的含量中等。In this Paper, glucosinolate composition and content in Chinese kale were studied. Glucosino-lates could be separated successfully in HPLC system with gradient eluents.Diode array detection (DAD) spectra showed that there was only one absorption peak at 225nm for aliphatic glucosinlates. However, the another absorption peak at 280nm was also observed in indole glucosinolate. 7 kinds of glucosinolates were detected Chinese kale.Gluconapin was the major gluconapin representing about 40%-60% of in the total amount.The content of 40H glucobrassicin and 4ME glucobrassicin were the lowest and there were moderate contents of glucoraphane, glucobrassicin and neoglucobrassicin in Chinese kale.

关 键 词：鉴定 HPLC 芥蓝 硫代葡萄糖甙 

分 类 号：S63[农业科学—蔬菜学] O657.72[农业科学—园艺学]