美兰对变形链球菌代谢的作用  

The effect of methylene blue on the metabolism of Streptococcus mutans

作　　者：王成龙[1] 李振纲[2] 赵皿[1] 吴可[3] 丛建波[3] 沈世杰[3] 

机构地区：[1]西安第四军医大学口腔医学院,710032 [2]北京中国人民解放军总医院 [3]军事医学科学院

出　　处：《实用口腔医学杂志》2002年第5期421-424,共4页Journal of Practical Stomatology

摘　　要：目的 :研究美兰对变形链球菌活性氧代谢及产酸代谢的作用 ,探讨美兰防龋的可行性。方法 :采用电子自旋捕捉法研究美兰对变形链球菌活性氧代谢的作用 ;采用气相色谱法研究美兰对变形链球菌产酸的抑制作用。结果 :美兰使变形链球菌糖代谢过程中产生的特征性的DMPO -O-·2 和DMPO -OH·ESR信号消失 ,并使得变形链球菌产酸总量显著减少。结论 :美兰对变形链球菌活性氧代谢和产酸代谢具有明显的抑制作用 ;Objective: To study the effect of methylene blue on the metabolism of reactive oxygen and acid production of Streptococcus mutans . Methods: Streptococcus of cricetusa, rattusb, mutansc, sobrinsd and mutans e,f and g were exposed to methylene blue at 2.5×10 -4 g/L for 1 h. The metabolism of reactive oxygen and acid production were detected by electron spin trapping method and gas chromatography respectively. Results: Electron spin resonance (ESR) spectrums of DMPO-O - · 2 and DMPO-OH · were observed in Streptococcus mutans cultured in glucose fluid, but were not found in those exposed to methylene. The total of acid production by Streptococcus mutans incubated with glucose + methylene blue were significantly lower than that with single glucose. Conclusion: Methylene blue may inhibit oxygen species metabolism and acid production of Streptococcus mutans , it may be used as a kind of dental caries prevention agent.

关 键 词：美兰 变形链球菌 代谢 活性氧 龋病 预防 

分 类 号：R780.2[医药卫生—口腔医学]