基因枪转化MAR序列介导水稻bar基因的表达分析  

Expression Analysis of Rice bar Gene Mediated Matrix Attachment Region(MAR) Sequence Transformed by a Gene Bombardment

作　　者：伍成祥[1] 宛煜嵩[2] 徐俊 苏金[4] 方宣钧[1] 

机构地区：[1]海南省热带农业资源开发利用研究所,三亚572025 [2]海南省农作物分子育种重点实验室,三亚572025 [3]海南科丰转基因生物研究中心,三亚572000 [4]中国农业科学院生物技术研究所,北京100081

出　　处：《农业生物技术学报》2002年第3期227-230,T001,共5页Journal of Agricultural Biotechnology

基　　金：国家863计划(2001AA211091);海南省自然科学基金项目(3003);2001年海南省科学事业费资助项目。

摘　　要：以水稻(OryzasativaL.)成熟胚愈伤组织作为水稻遗传转化受体,采用带有MAR(matrixattachmentregion)和不带MAR的两种植物表达质粒进行基因枪轰击转化bar基因,获得抗basta转基因水稻。分析了MAR序列介导在转基因水稻中对转基因表达的影响。研究结果表明,利用MAR序列介导bar基因表达,获得的转基因系的数量比对照提高66.7%,单块抗性愈伤组织再生植株数比对照提高27.3%。MAR序列介导bar基因表达的转基因系中,bar基因的表达水平比对照高,并且不同转基因系间bar基因表达差异比对照小。Employing embryogenic calli induced from mature embryo scutum of rice (Oryza sativa L.) as acceptors, the authors set up a gene bombardment mediated rice genetic transformation system and acquired transgenic plants that were resistant to basta. Effects of MARs (matrix attachment regions) on bar gene expression in transgenic rice were studied with two special plant expression plasmids. The result indicated as follows: In the genetic transformation of rice with MARs mediated bar gene, the number of transgenic lines was 66.7 % higher than that of the control, and the number of transgenic plants regenerated from each resistant callus was also increased by 27.3%. In the lines that bar gene were mediated by MARs, expression level of bar gene was higher than that of the control, and expression variance of bar gene among transgenic lines was smaller as well.

关 键 词：基因枪转化 MAR序列 水稻 BAR基因 表达分析 转基因表达 

分 类 号：S511.03[农业科学—作物学]