Cre重组酶结构与功能的研究进展  

Progress in the Study of the Structure and Function of Cre Recombinase

作　　者：王立霞[1] 王勇[1] 胡鸢雷[1] 高音[1] 林忠平[1] 

机构地区：[1]北京大学蛋白质工程及植物基因工程国家重点实验室,北京100871

出　　处：《生物工程学报》2002年第5期532-535,共4页Chinese Journal of Biotechnology

基　　金：国家 8 6 3高技术研究项目基金资助 (No .0 10 0 4 0 3 0 1)~~

摘　　要：Cre loxP定位重组系统来源于噬菌体P1 ,由Cre重组酶和loxP位点两部分组成。在Cre重组酶的介导下 ,设定的DNA片段可以被切除 ,可以发生倒位 ,亦可造成定点的整合。由于其作用方式高效简单 ,Cre loxP定位重组系统已在特定基因的删除、基因功能的鉴定、外源基因的整合、基因捕获及染色体工程等方面得到了有效的利用 ,在转基因的酵母、植物、昆虫、哺乳动物的体内外DNA重组方面成为一个有力的工具。这里就Cre重组酶的结构。The Cre recombinase, an integrase from bacteriophage P-1, catalyzes site-specific recombination between 34-bp repeats termed loxP sites, in the absence of any additional cofactors and energy. Mediated by Cre recombinase, specific DNA fragments can be excised, inversed or integrated depending on the orientation or position of loxP sites in vitro or in vivo. Because of its simplicity and high efficiency, Cre/loxP site-specific recombination system has been widely used in gene deletion and function identification, gene site-specific integration, gene trapping and chromosome engineering. It has been used as a useful tool for DNA recombination in transgenic yeast, plants, insects and mammals. Here progress in the study of the structure and function of Cre recombinase is discussed.

关 键 词：CRE重组酶 结构 功能 研究进展 LOXP位点 定位重组 

分 类 号：Q55[生物学—生物化学] Q784