M_2 muscarinic receptor of spinal cord mediated increase of nNOS expression in locus coeruleus during morphine withdrawal  

吗啡戒断时M_2毒蕈碱受体介导大鼠蓝斑核中nNOS表达增强(英文)

作　　者：丑维斌[1] 曾因明 段世明[1] 周文华[2] 顾钧[2] 杨国栋[2] 

机构地区：[1]江苏省重点麻醉学实验室,徐州医学院徐州221002 [2]宁波戒毒研究中心,宁波中国315010

出　　处：《Acta Pharmacologica Sinica》2002年第8期691-697,共7页中国药理学报（英文版）

摘　　要：AIM: To investigate the effects of different muscarinic receptor (M) subtypes in the spinal cord on the scores of naloxone-precipitated morphine-withdrawal symptoms and the changes of nNOS expression in locus coeruleus (LC). METHODS: nNOS immunohistochemistry, intrathecal injection (it), and antisense oligonucleotides (AS-ONs) techniques were used. RESULTS: Intrathecal injection of M2-antisense oligonucleotides (M2-AS) decreased the scores of morphine withdrawal symptoms. M1-AS attenuated morphine-withdrawal symptoms, but the effect was less than that of M2-AS. The expression of nNOS positive neurons in the LC increased in morphine-dependent rats and increased to a greater extent during morphine withdrawal. Intrathecal injection of M2-AS inhibited the increase of nNOS expression in LC during morphine-withdrawal, but there was no effect in case of M1-AS. CONCLUSION: M2 muscarinic receptor of spinal cord mediated the increase of nNOS expression in LC during morphine withdrawal.目的:观察脊髓不同M受体亚型对纳络酮催促吗啡戒断大鼠戒断症状评分以及蓝斑核nNOS表达变化的影响.方法:采用nNOS免疫组织化学、鞘内注射和反义寡核苷酸技术.结果:鞘内注射M_2-AS可显著减少吗啡戒断症状评分,M_1-AS虽可部分减轻戒断症状,但总的作用弱于 M_2-AS;吗啡依赖大鼠蓝斑核nNOS表达增加,用纳络酮催促戒断后nNOS表达进一步增加,鞘内注射M_2-AS可抑制在吗啡戒断时蓝斑核nNOS的表达增强,而M_1-AS对其无影响.结论:脊髓M_2受体介导吗啡戒断大鼠蓝斑核nNOS的表达增加.

关 键 词：MORPHINE substance withdrawal syndrome muscarinic receptors nitric-oxide synthase spinal cord locus coeruleus 

分 类 号：R595.5[医药卫生—内科学]