新辅助性原位部分肝移植动物模型的建立  

Establishment of a new pig model for auxiliary partial orthotopic liver transplantation

作　　者：彭承宏[1] 史留斌[1] 彭淑牖[1] 何伟[1] 刘颖斌[1] 徐斌[1] 陈晓鹏[1] 白明东[1] 王涌[1] 唐喆[1] 

机构地区：[1]浙江大学医学院附属第二医院外科,杭州310009

出　　处：《中华医学杂志》2002年第15期1054-1056,共3页National Medical Journal of China

基　　金：浙江省科技计划项目基金资助 ( 0 0 1110 2 32 )

摘　　要：目的　建立一种新的猪辅助性部分原位肝移植模型。方法　将供肝左叶在体外修肝时切除 ,右叶供肝作为移植肝。切除受肝左外叶 ,右叶供肝肝上下腔静脉与受肝肝上下腔静脉行端侧吻合 ,供肝门静脉与受肝门静脉行端侧吻合 ,受体脾动脉在结肠后与供体肝动脉端端吻合 ,胆总管置管外引流。结果　共进行移植模型 5例 ,成功 4例 ,成功率达 80 % ,模型血流动力学稳定。结论　本移植术式在体外转流下行全肝血流阻断 ,门静脉淤血明显减轻 ,阻断时间缩短 ,可以有效防止受体肝的缺血再灌注损伤。血管处理简单 ,吻合质量高。术后出血少。成功率高 。Objective To establish a new pig model for auxiliary partial orthotopic liver transplantation (APOLT). Methods The liver of the donor was removed from its body. The left lobe of the liver was resected in vitro, and the right lobe was used as a graft. After the left lateral lobe of the recipient was resected, end-to-side anastomoses were performed between the suprahepatic inferior venae cavae of the donor and between the portal veins of the donor liver and those of recipient liver respectively. End-to-end anastomosis was performed between the hepatic artery of graft and the splenic artery of host. Outside drainage was placed in the donor′s common bile duct. Results Model of APOLT was established in 5 pigs with a success rate of 80%. The hemodynamics of the recipient was stable during and after operation. Conclusion Significant decrease of congestion in portal vein and shortened blocking time were obtained because of the application of veno-venous bypass in vitro during complete vascular clamping. This new procedure, with such advantages as simple vessel processing, quality anastomosis, less postoperative hemorrhage and higher success rate, etc, effectively prevents ischemical reperfusion injury of the host liver and deserves to be spread.

关 键 词：辅助性部分原位肝移植 模型 猪 

分 类 号：R-332[医药卫生]