半边旗二萜类化合物5F对人翼状胬肉成纤维细胞增殖周期和Ki-67表达的影响  

The effect of diterpenoid compound 5F of Peris semipinnata L on the changes of the cell cycle and expressions of Ki-67 in fibroblasts of human pterygium in vitro

作　　者：陈毓东[1] 王映芬[1] 

机构地区：[1]广东医学院附属医院眼科,广东湛江524001

出　　处：《眼科》2002年第5期305-307,I008,共4页Ophthalmology in China

摘　　要：目的 :观察中药半边旗二萜类化合物 5F对体外培养的人翼状胬肉成纤维细胞 (HPF)增殖周期和Ki 6 7表达的影响。方法 :用流式细胞仪 (FCM)分析不同浓度 5F对HPF周期分布、免疫细胞化学法 (ICH)检测 5F对增殖指数Ki 6 7抗原的影响。结果 :用药后HPF停滞于G1期 ,S、G2 /M期细胞减少 ;Ki 6 7阳性表达率对照组为 (2 6 16± 1 2 6 ) % ,5F浓度 8μg/ml时下降 ,在 12 8μg/ml时降至 (4 10± 0 6 3) %。各用药组与对照组比较均有显著性差异 (P <0 0 1)。各用药组间两两比较有显著性差异 (P <0 0 1)。结论 :5F对HPF增殖的抑制作用与细胞周期、Ki 6Objective:To observe the effect of diterpenoid compound 5F of Peris semipinnata L on the proliferative cell cycle and expressions of Ki-67 in cultured human pterygium fibroblasts(HPF).Methods:The changes of the cell cycles of HPFs were evaluated with flow cytometry (FCM) and the expression of Ki-67 was determined with SP method.Results:5F arrested most of cells at the end of G1 stage,and the cells in S and G2/M phases were gradually diminished.Immunocytochemical stain showed that the expression of Ki-67 in HPFs was (26.16±1.26)% in control groups,decreased at the concentration of 8 μg/ml of 5F,and diminished to (4.10±0.63)% at 128μg/ml.The differences were significant between these groups ( P <0.01).Conclusion:The inhibitory effect of 5F on HPFs may due to the arrest in cell cycle and the decreased expression of Ki-67.

关 键 词：成纤维细胞 半边旗 细胞周期 翼状胬肉 免疫学 中药 免疫细胞化学法 流式细胞仪 

分 类 号：R777.33[医药卫生—眼科]