十一丙酸睾酮对胃上皮细胞株生物学特性的影响  

Effects of testosterone undecanoate on the growth of human gastric cell lines

作　　者：黄海力[1] 吴本俨[1] 徐世平[1] 

机构地区：[1]中国人民解放军总医院南楼消化科,北京市100853

出　　处：《世界华人消化杂志》2002年第10期1125-1128,共4页World Chinese Journal of Digestology

摘　　要：目的:观察十一丙酸睾酮对胃癌细胞株SGC-7901以及正常胃上皮细胞株HFI-145生长的影响方法:用不同浓度的十一丙酸睾酮作用细胞,MTT方法研究生长状况,FCM观察细胞周期分布,TUNEL法检测细胞凋亡.比较不同浓度作用组间差异以及与对照组的差异.结果:0.0004-0.4μmol/L十一丙酸睾酮对SGC-7901细胞生长有促进作用,对HFI-145细胞作用不明显;4μmol/L浓度对两种细胞均产生抑制,有时间依赖性,作用3d抑制率分别达到17%和27%,4μmol/L用药组细胞的G0/G1期细胞较对照组增多,FCM以及TUNEL未发现凋亡百分比的改变.结论:高浓度十一丙酸睾酮通过阻滞细胞于G0/G1期抑制胃上皮细胞生长,低浓度促进胃癌上皮细胞生长可能对胃癌的发展及预后产生影响.AIM:to investigate the effects of the anbolic-androgenic ste-roids testosterone undecanoate (TU) on the growth of hu-man normal gastric cell HFI-145 and gastric cancer cell lineSGC-7901METHODS:MTT colorimetric assay was used to measurethe proliferation of HFI-145 and SGC-7901 cells exposed todifferent concentration of TU. Apoptosis and cell cycle phasedistribution were measured by TUNEL and flow cytometricanalysis between the 4μmol/L TU treated group and non-treated group.RESULTS:Compared with non-treated group, TU from0.0004 to 0.4μmol/L exerted no action on HFI-145 cell buthad a stimulating effect on SGC-7901 cell lines. High con-centration of TU (4μmol/L) had an inhibitory effect on theproliferation of both cells by inducing them to G0/G1 arrestat a time-depending manner, which peaked at 72 h.Themaximum inhibitory rate was 17% and 27% respectively.CONCLUSIONS:High concentration of TU inhibits the growthof human gastric cells by arresting the cell cycle progres-sion in G0/G1 phase but physiologic concentration of TUhas a stimulating effect on SGC-7901 cell lines.

关 键 词：十一丙酸睾酮 胃上皮细胞 生物学特性 胃癌 雄激素 相关性 

分 类 号：R735.2[医药卫生—肿瘤]