MicroRNA-34c-5p在喉癌前病变组织中的特征性差异表达及临床病理分析  

Expression of MicroRNA-34c-5p in laryngeal epithelial premalignant lesions and clinicopathological analysis

作　　者：胡艳萍[1,2] 武迎[1] 魏丽[1] 金玉兰[1] 李雪[1] 刘红刚[1] 

机构地区：[1]首都医科大学附属北京同仁医院病理科,头颈部分子病理诊断北京市重点实验室,北京100730 [2]首都医科大学附属北京潞河医院病理科,北京101149

出　　处：《中国耳鼻咽喉头颈外科》2015年第8期405-410,共6页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：高等学校博士学科点专项科研基金(20131107110004);2014年度科技创新基地培育与发展工程专项项目(Z141107004414028)联合资助

摘　　要：目的探讨micro RNA-34c-5p(mi R-34c)在喉癌前病变组织中差异表达情况,并进行临床病理相关性分析。方法按照2005年世界卫生组织(WHO)病理诊断标准,94例喉癌前病变分为轻度异型增生、中度异型增生、重度异型增生及原位癌;实时定量聚合酶链式反应(quantitative real-time polymerase chain reaction,q RTPCR)方法检测94例喉癌前病变组织中mi R-34c在各组间的差异表达情况,并结合临床资料及随访结果分析mi R-34c与喉癌前病变病例临床因素的相关性。结果 94例喉癌前病变病例年龄37~86岁,平均58岁,男性85例(90.4%),女性9例(9.6%)。轻度异型增生组20例(21.3%),中度异型增生组23例(24.5%),重度异型增生组26例(27.7%),原位癌组25例(26.6%)。随喉癌前病变级别增高,mi R-34c在喉癌前病变组织中呈上调表达趋势,总体组间存在统计学差异(F=10.182,P=0.00),而在浸润性鳞状细胞癌组下降,甚至明显低于正常鳞状上皮组织的表达均值。临床病理分析显示饮酒病例中表达明显高于非饮酒病例,且mi R-34c与喉癌前病变患者饮酒因素存在明显线性依存关系(r=0.31,P=0.03)。结论 Mi R-34c在喉癌前病变组织中的上调表达趋势,可能为辅助喉癌前病变病理分级和早期喉癌诊断提供一定的分子病理参考价值,mi R-34c的上调表达与喉癌前病变饮酒因素呈线性相关,对于指导喉癌前病变病例的临床管理可能具有重要临床意义。OBJECTIVE To investigate the expression of miR-34 c in laryngeal epitheliual premalignant lesions and to analyze the correlations between its dysregulation with clinicopathological parameters of laryngeal epitheliual premalignant lesions.METHODS According to 2005 WHO classification system,94 cases of laryngeal epitheliual premalignant lesion were grouped into mild dysplasia,moderate dysplasia,severe dysplasia and carcinoma in situ.The expression of miR-34 c in 94 cases of laryngeal epitheliual premalignant lesion was detected by qRT-PCR,and the correlation of clinicopathological parameters with the clinical and follow-up data of all laryngeal epitheliual premalignant lesions were analyzed.RESULTS The average age of 94 patients with laryngeal epitheliual premalignant lesion was 58 years(ranged from 37 to 86years).Distribution across the gender was 85 males(90.4%)and 9 females(9.6%).All laryngeal epitheliual premalignant lesions were summarized as follows:20(21.3%) with mild dysplasia,23(24.5%) with moderate dysplasia,26(27.7%)with severe dysplasia,and 25(26.6%) with carcinoma in situ.With the increased grading of dysplasia,miR-34 c was upregulated in laryngeal epitheliual premalignant lesions with significant statistical difference among all groups(F=10.182,P=0.00),While the expression of miR-34 c was sharply dropped in saquamous cell carcinoma(SCC) group and even lower than that in normal tissue(NOR) group.On the univariate analysis,miR-34 c expression level in laryngeal epitheliual premalignant lesion of alcohol drinking patients was higher than that of non-drinkers.The expression had a significant linear regression relationship with the alcohol drinking(r=0.31,P=0.03).CONCLUSION Up regulation of miR-34 c may be helpful for grading of laryngeal epitheliual premalignant lesions and diagnosis of early laryngeal cancer as a valuable molecular markers,and its linear correlation with alcohol drinking of laryngeal epitheliual premalignant lesions showed important guidance implication in clinical management of laryngea

关 键 词：喉肿瘤 癌前状态 微RNAS 实时定量PCR 

分 类 号：R739.65[医药卫生—肿瘤]