氧化修饰的低密度脂蛋白对内皮细胞功能的影响  

The functional effect of oxidized low density lipoprotein on endothelial cells

作　　者：周娟[1] 王竹青 雷立权[1] 刘进军[1] 

机构地区：[1]西安交通大学医学院病理生理学教研室,西安710061 [2]西安市庆安医院心血管内科,西安710077

出　　处：《西安交通大学学报（医学版）》2002年第5期432-434,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的　观察OX LDL对内皮细胞NO、ET 1合成及细胞表面细胞间粘附分子 1(ICAM 1)表达的影响。方法　采用培养的人脐静脉内皮细胞 (HUVEC) ,在分别加入不同浓度OX LDL(5 0、10 0、15 0mg·L-1)孵育 2 4h ,或浓度为 10 0mg·L-1于不同时间 (12、2 4、36h)孵育后观察培养液中NO及ET 1水平及细胞表面ICAM 1的表达。结果　OX LDL可显著抑制NO的合成 (P <0 .0 5 ) ,但各时间段培养液中NO含量无显著性差异 (P >0 .0 5 ) ;OX LDL还可明显增加ET 1的分泌及诱导细胞表面ICAM 1的表达。结论　OX LDL对内皮细胞具有明显的细胞毒作用 ,它对NO、ET 1释放及ICAM 1表达的影响可能是其致动脉粥样硬化发生的重要机制。Objective To investigate the effect of oxidized low density lipoprotein on the synthesis of NO, ET-1 and expression of ICAM-1 in human umbilical vein endothelial cells (HUVEC). Methods OX-LDL of 50?mg·L -1, 100?mg·L -1 and 150?mg·L -1 were co-incubated with HUVEC for 24?h in vitro. OX-LDL 100?mg·L -1 were co-incubated with HUVEC for 12 hours, 24 hours or 36 hours respectively. The levels of NO, ET-1 in medium and the surface expression of ICAM-1 on HUVEC were detected after the experiments.Results OX-LDL could significantly inhibit the synthesis of NO (P< 0.05), but at every interval the differences of NO level were not significant (P> 0.05); OX-LDL could increase the secretion of ET-1 and induced ICAM-1 upregulation of surface expression on HUVEC.Conclusion OX-LDL have cytotoxic effects on HUVEC. The influence of OX-LDL on the release of NO, ET-1 and the expression of ICAM-1 on HUVEC may be the crucial mechanism which accounts for its atherogenic effects.

关 键 词：氧化修饰 低密度脂蛋白 内皮细胞 一氧化氮 内皮素-1 细胞间粘附分子-1 动脉粥样硬化 

分 类 号：R363.2[医药卫生—病理学] R543.5[医药卫生—基础医学]