全反式维甲酸联合猪胆酸钠对人卵巢癌细胞生长抑制作用的影响  

The inhibitory effects of all-trans retinoic acid plus swine bile acid sodium salt on the proliferation and induction of differentiation in human ovarian cancer cell lines

作　　者：刘琦[1] 刘春敏[1] 任蕴芳[2] 孟奎[1] 吴波[1] 

机构地区：[1]南京军区南京总医院,南京大学临床医学院妇产科210002 [2]中国军事医学科学院基础医学研究所

出　　处：《江苏医药》2002年第11期822-824,共3页Jiangsu Medical Journal

摘　　要：目的　探讨维甲酸 (ATRA)及猪胆酸钠 (SBANa)联合应用对人卵巢癌细胞生长的抑制作用。方法　实验共分为三组 :单用ATRA组、单用SBANa组及ATRA +SBANa联合用药组。应用MTT比色法、流式细胞仪对COC1、COC2 、CAOV3 三种卵巢癌细胞系在不同浓度的ATRA及SBANa下进行检测。结果　ATRA及SBANa联合应用后 ,能增强抑制卵巢癌细胞生长作用 ,当达到ATRA10 μmol/L +SBANa 10 0 μg/ml浓度时 ,G0 、G1期细胞比例增加 ,G2 M、S期细胞比例下降 ,细胞增殖速度较单独用药组减缓。MTT检测提示在联合用药浓度达到ATRA 10 μmol/L +SBANa 10 0 μg/ml时 ,癌细胞抑制率最佳。结论　ATRA及SBANa联合应用可增强对卵巢癌细胞增殖的抑制作用 ,并在ATRA 30 μmol/L +SBANa 10 0 μg/ml浓度时 ,促进癌细胞凋亡。Objective To investigate the inhibitory effects of the combination of all-trans retinoic acid(ATRA) and swine bile acid-sodium(SBANa) on proliferation and induction of differentiation in human ovarian carcinoma cell lines COC_1,COC_2 and CAOV_3 in vitro.Methods Each of the three lines was divided into three treatment groups(regimen A:ATRA alone,regimen B:SBANa alone,regimen C:ATRA plus SBANs).The proliferation of cancer cells lines was evaluated respectively at different drug concentrations by the methodology of tetrazolium-based colorimetric assay(MTT assay);cell cycles were assessed by flow cytometry and morphological changes of the cells were observed by inverted microscopy.Results ATRA plus SBANa showed the synergistic inhibitory effects on the cellular growth and induction of differentiation in all the three-line cells in a dose-dependent manner within a certain concentration range(ATRA≤30μmol/L;SBANa≤150μg/ml).In the regimen C(ATRA 10μmol/L plus SBANa 100μg/ml)the cancer cell population in phase G_0/G_1 increased,but cell in G_2M/S reduced and thus proliferation index(PI)decreased as compared with regimen A and B.At the concentration of ATRA 30μmol/L plus SBANa 100μg/ml,the growth inhibitory rate for the three-line cells was highest.Conclusion It is suggested in the present study that ATRA plus SBANa had more inhibitory effect than ATRA alone on human ovarian cancer cells in vitro and induced more obvious cancer cell apoptosis.

关 键 词：全反式维甲酸 猪胆酸钠 卵巢癌 细胞生长 抑制作用 实验研究 

分 类 号：R737.31[医药卫生—肿瘤]