侧脑室微量注射神经生长因子对实验性抑郁症大鼠抑郁行为和海马神经元损伤的影响  

The influence of introcerebralventricular injection of nerve growth factor on the depressive behavior and injury of hippocampal neurons in an experimental depression model of rats

作　　者：王雪琦[1] 路长林[1] 孙学军[1] 赵小林[1] 何成[1] 

机构地区：[1]第二军医大学神经生物学教研室,上海200433

出　　处：《中国行为医学科学》2002年第5期481-484,共4页Chinese Journal of Behavioral Medical Science

基　　金：全军医学科研"十五"计划青年基金项目 0 1Q0 94;全军医学科研"十五"计划重点基金项目 0 1Z0 65;国家自然科学基金重点项目 39930 0 80资助

摘　　要：目的　观察中枢给予外源性NGF对实验性抑郁症大鼠抑郁行为和海马神经元损伤的影响。方法　采用长期不可预见性中等强度应激造成大鼠抑郁症模型 ,运用侧脑室微量注射技术、Openfield行为测定、组织化学、光镜和电镜技术观察中枢给予外源性NGF对实验性抑郁症大鼠抑郁行为和海马神经元损伤的影响。结果　侧脑室微量注射NGF可显著改善实验性抑郁症大鼠的抑郁行为 ,使实验性抑郁症大鼠海马CA1、CA3及齿状回 (DG)神经元数目显著增加 ,酸性磷酸酶活性显著降低 ,明显改善海马神经元的超微结构。结论　外源性给予NGF可能通过减轻大鼠海马神经元的损伤 。Objective To observe the effects of i.c.v. NGF on the depressive behavior and changes of the hippocampal neurons in the depressive rats. Methods The depression model of rats was established by chronic unpredictable mild stress. The techniques of intracerebralventricular microinjection, openfield testing, histochemistry, light microscope and electron microscope were used to observe the effects of i.c.v. NGF on the depressive behavior and changes of the morphological and number of hippocampal neurons in the depressive rats. Results Compared with the i.c.v. saline , i.c.v. NGF could improve the depressive behavior significantly, mitigate the decrease of number of hippocampal neurons, decrease the activity of ACP in hippocampal neurons and the morphological changes of hippocampal neurons were ameliorated. Conclusion I.c.v. NGF might improve depressive behavior by protecting the hippocampal neurons from the injuries induced by experimental depression.

关 键 词：抑郁症 大鼠 神经生长因子 行为 海马神经元 

分 类 号：R749.4[医药卫生—神经病学与精神病学]