杭白菊对小牛血管平滑肌细胞凋亡及其抗氧化性研究  

Effect of Chrysanthemum morifolium Ramat on apoptosis of bovine aortic smooth muscle cells

作　　者：方雪玲[1] 王锡田[1] 黄淑如[1] 李霞[1] 

机构地区：[1]浙江大学医学院附属第一医院,浙江杭州310003

出　　处：《浙江大学学报（医学版）》2002年第5期347-350,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省中医药局课题 (2 0 0 2 C0 77)

摘　　要：目的 :探讨杭白菊提取液对小牛主动脉血管平滑肌细胞 (VSMC)凋亡的影响及可能的作用机制。方法 :取小牛主动脉进行体外 VSMC培养 ,加入杭白菊提取液干预 ,用膜联蛋白 Annexin检测法 ,在流式细胞仪下观察VSMC的凋亡现象 ,同时取上清液在分光光度仪下测定超氧化物歧化酶 (SOD)、丙二醛 (MDA)。结果 :1在流式细胞仪下观察到加入杭白菊提取液后 VSMC凋亡数量减少 ,随着杭白菊提取液浓度的加大 ,细胞凋亡从 (4.4 2 5±0 .6 2 4 ) %降至 (2 .875± 0 .6 4 0 ) % ;2随着杭白菊提取液浓度的增加 ,SOD值由 (1.6 83± 0 .149)× 10 4 U/ L升高到(2 .2 97± 0 .2 30 )× 10 4 U/ L、MDA值由 (16 6 .4 5 4± 5 6 .80 5 ) μmol/ L下降至 (73.0 6 8± 2 7.2 0 3) μmol/ L。结论 :杭白菊提取液具有抑制小牛 VSMC凋亡及抗氧化作用 ,并呈浓度依赖性。Objective: To observe the effect and the mechanism of Chrysanthemum morifolium Ramat on apoptosis of bovine aortic smooth muscle cells. Methods: Vascular smooth muscle cells were isolated from thoracic aorta of fetal calf and cultured, then incubated with different concentration of Chrysanthemum morifolium Ramat. Apoptosis was measured by flow cytometry. SOD and MDA were measured by spectrophotometer. Results: We found that: ① the number of apoptotic cells was reduced from (4.425±0.624)% to (2.875±0.640)% in Chrysanthemum morifolium Ramat group, in a concentration dependent manner; ② the value of SOD was increased from ( 1.683 ±0.149)×10 4 U/L to (2.297±0.230)×10 4 U/L and the value of MDA was reduced from (166.454± 56.805 )μmol/L to (73.068±27.203)μmol/L in Chrysanthemum morifolium Ramat group, also in a concentration dependent manner. Conclusion: Chrysanthemum morifolium Ramat can inhibit apoptosis of vascular smooth muscle cells in a concentration-dependent manner.

关 键 词：杭白菊 血管平滑肌细胞凋亡 抗氧化性 脱噬作用 自由基 

分 类 号：R285.5[医药卫生—中药学]