硫代反义脱氧寡核苷酸对脑胶质瘤SWO-38细胞的作用  

Effects of phosphorothioate antisense α1,4Gal T oligonucleotide on human gliomas cell line SWO-38

作　　者：郑刚[1] 张积仁[1] 刘发全[1] 王雄文[1] 张新宇[2] 

机构地区：[1]第一军医大学珠江医院肿瘤科,广州510282 [2]第一军医大学珠江医院骨科,广州510282

出　　处：《广东医学》2002年第10期1020-1022,共3页Guangdong Medical Journal

摘　　要：目的　研究α1,4半乳糖糖基转移酶 (α1,4GalT)基因硫代反义核酸对脑胶质瘤细胞系SWO -3 8细胞的增值与凋亡的影响及其与Bcl -2和Bax表达的关系。方法　采用脂质体介导的基因转染方法将人工合成α1,4GalT基因反义核酸转入SWO -3 8细胞中。应用RT -PCR检测对α1,4GalTmRNA表达的影响 ,应用克隆形成实验检测对细胞增殖的作用 ,应用流式细胞术 (FCM)和琼脂糖凝胶电泳检测细胞凋亡的发生 ,应用FCM检测Bax和Bcl -2表达的变化。结果　反义核酸转染后 ,经RT -PCR检测证实α1,4GalTmRNA基因的表达下降 ;克隆形成试验显示细胞生长明显受到抑制 (P <0 .0 1) ;FCM检测表明 ,导入α1,4GalT基因反义核酸的SWO -3 8细胞的凋亡明显增加 (P <0 .0 1) ,Bcl -2蛋白表达显著下降 (P <0 .0 1) ,而Bax蛋白表达显著提高 (P <0 .0 1)。结论　α1,4GalT基因硫代反义核酸可诱导脑胶质瘤细胞系SWO -3 8细胞凋亡 。Objective To study the effects of phosphorothioate antisense α1,4Gal T oligonucleotide on human gliomas cell line SWO-38. Methods SWO38 gliomas cells were reacted with 10 μmol/L phosphorothioate antisense α1,4Gal T oligonucleotide for 72 hours by means of lipofectin transfection. The growth inhibition was detected by colony-forming unit assay. Analysis of DNA fragmentation was examined by flow cytometric analysis(FCM). The expressions of Bcl-2 and Bax protein were determined by FCM.Results Phosphorothioate antisense α1,4Gal T oligonucleotide can signifantly inhibite the growth of gliomas cells( P< 0.01), induce apoptosis of gliomas cell line SWO-38( P< 0.05), downregulate expression of Bcl-2 protein and upregulate expression of Bax protein greatly( P< 0.01). Conclusion Phosphorothioate antisense α1,4Gal T oligonucleotide can significantly decrease proliferation and induce apoptosis of human gliomas cell line SWO-38,which maybe related to regulated expressions of Bcl-2 and Bax.

关 键 词：硫代反义脱氧寡核苷酸 脑胶质瘤 α1 4半乳糖糖基转移酶 BCL-2基因 BAX基因 基因转染 

分 类 号：R739.41[医药卫生—肿瘤]