电针对戊四唑点燃型癫痫大鼠脑内氨基酸含量的影响  

Effect of Electroacupuncture on the Contents of Amino Acids in the Brain in PTZ Kindled Epilepsy Rats

作　　者：杨帆[1] 徐国龙[1] 王频[2] 施瑾[1] 章复清[1] 昂文平[1] 沈德凯[1] 刘向国[1] 

机构地区：[1]安徽中医学院针灸经络研究所,合肥230038 [2]安徽中医学院临床医学二系

出　　处：《针刺研究》2002年第3期174-176,共3页Acupuncture Research

基　　金：安徽省教育厅自然科学基金项目 (No.1999JL139);安徽省教育厅青年教师科研基金项目部分 (No.2 0 0 0QL148)

摘　　要：目的 :探讨戊四唑 (PTZ)点燃型癫痫大鼠脑内氨基酸的变化及电针对其影响。方法 :成年SD大鼠 3 0只随机分为三组 (每组 1 0只 ) :癫痫模型组、电针组、正常对照组。采用氨基酸自动分析仪测定脑内氨基酸含量。结果 :癫痫模型组脑内兴奋性氨基酸谷氨酸 (Glu)及抑制性氨基酸甘氨酸 (Gly)升高明显 (P <0 .0 5) ;而抑制性氨基酸γ 氨基丁酸 (GABA)降低明显 (P <0 .0 1 )。经电针治疗后γ 氨基丁酸 (GABA)、丙氨酸 (Ala)含量明显升高 (P <0 .0 1、P <0 .0 5) ,而Glu降低明显 (P <0 .0 5)。结论 :电针抗癫痫的作用可能与脑内Glu。Objective: To observe the changes of amino acids in the brain of pentylenetetrazole(PTZ) kindled epilepsy rats and to explore the effect of electroacupuncture (EA). Methods: 30 adult SD rats were randomly divided into three groups (10 rats in each group): control group, epilepsy model group and EA group. Epilepsy rat model was established by intraperitoneal injection of PTZ (32 mg/kg), once daily, continuously for 25 days. EA treatment was given to the rat by puncturing and electrically stimulating 'Baihui' (GV 20) and 'Fengchi' (GB 20) for 30 min (100 Hz, 6 mA), once daily, continuously for 7 days. Contents of aspartic acid (ASP), glutamic acid (Glu), glycine (Gly), γ-aminobutyric acid (GABA) and alanine (Ala) in the brain tissue supernatant were measured with 835-50 Type Amino-acid Automatic Analysis Apparatus. Results: In epilepsy model group, ASP, Glu and Gly contents increased , while GABA and Ala levels decreased in comparison with those of control group. Among them, the increase of Glu (excitatory amino acid) and Gly (inhibitory amino acid glycine) and the decrease of GABA were remarkable (P<0.05～0.01). After EA treatment, the contents of ASP, Glu and Gly presented a decreasing tendency, while those of GABA and Ala increased considerably compared with model group(P<0.01,P<0.05),and the decrease of Glu level was significant (P<0.05).Results indicated that EA could lessen the contents of excitatory amino acids and raise those of some inhibitory amino acids in the brain tissue of epilepsy rats. Conclusion: The effect of EA in treatment of epilepsy is likely related with EA generated changes of excitatory and inhibitory amino acids levels in the brain tissues.

关 键 词：癫痫 氨基酸 电针 治疗 

分 类 号：R277.721[医药卫生—中医学] R245.97