NQO1和GSTT1基因多态性与慢性苯中毒的危险性  

Relation of genetic polymorphism of NQO1 and GSTT1 with risks of chronic benzene poisoning

作　　者：万俊香[1] 施锦绣[2] 管继如[3] 叶榕[3] 高晓玲[4] 刘薇薇[5] 惠利健[2] 曹多志 金锡鹏[1] 胡赓熙[2] 夏昭林[1] 

机构地区：[1]复旦大学公共卫生学院职业卫生室,上海200032 [2]中国科学院上海细胞所,200031 [3]杭州市疾病控制中心 [4]上海市第一人民医院 [5]广州市职业病防治院 [6]马鞍山钢铁公司医院劳动卫生研究所

出　　处：《中华劳动卫生职业病杂志》2002年第5期340-343,T001,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：卫生部青年科研基金项目 (96Q0 3 0 );教育部回国人员资助项目(1999);国家自然科学基金项目 (3 0 2 71113 )

摘　　要：目的　探讨NQO1和GSTT1的基因多态性和苯中毒易感性的关系。方法　采用病例 -对照研究 ,以 15 2名苯中毒工人为病例组 ,15 2名接触苯而没有中毒表现的工人为对照组。采用多聚酶链反应 (PCR)、变性高效液相色谱 (DHPLC)和测序检测NQO1基因的启动子和全部编码区的单核苷酸多态性 (singlenucleotidepolymorphism ,SNP) ,以多重PCR检测GSTT1的基因型。结果　在经常吸烟的人群中 ,携带NQO1c .6 0 9T/T基因型的个体接触苯时发生慢性苯中毒的危险性是C/C和C/T基因型的 7.73倍 (95 %CI:1.71～ 34.97,P =0 .0 10 )。在经常饮酒的人群中 ,携带NQO1第 6外显子T/T突变纯合子的个体在接触苯时发生慢性苯中毒的危险性是C/C和C/T基因型的 11.0 0倍 (95 %CI:1.89～ 6 3.83,P =0 .0 0 5 )。结论　携带NQO1c .6 0 9T/T基因型而又同时吸烟或饮酒的个体对苯中毒可能易感。Objective To explore the relation between genetic polymorphisms of NQO1,GSTT1 and risks of chronic benzene poisoning(BP). Methods A case-control study was conducted.152 BP patients and 152 workers occupationally exposed to benzene without poisoning manifestations were investigated.Polymerase chain reaction(PCR),denaturing high-performance liquid chromatography(DHPLC) and sequencing were used to detect the single nucleotide polymorphisms(SNPs) of the promoter and complete coding-region of NQO1 gene.Multiple PCR was used to detect GSTT1 genotype. Results In smoking population,there was 7.73-fold(95%CI:1.71～34.97,P=0.010) of risk in BP subjects carrying NQO1c.609 T/T genotype,compared with those carrying C/C and C/T.genotype.In drinking population,the individuals carrying the 6th extron of NQO1c.609 T/T homozygote genotype had a 11.00-fold(95%CI:1.89～63.83,P=0.005) risk of BP compared to those with NQO1c.609 C/T and C/C genotypes. Conclusion The subjects carrying NQO1c.609 T/T genotype and together with the habit of smoking or drinking may be more susceptible to BP.

关 键 词：NQO1 GSTT1 危险性 苯中毒 基因多态性 生活方式 

分 类 号：R135.12[医药卫生—劳动卫生]