吡虫啉和抑食肼对人体外周血淋巴细胞遗传物质的影响  

EFFECT OF IMIDACLOPRID AND RH-5849 ON GENETIC MATERIAL OF HUMAN PERIPHERAL LYMPHOCYTES

作　　者：封少龙[1] 孔志明[2] 王五香 王新明[1] 彭平安[1] 

机构地区：[1]有机地球化学国家重点实验室 [2]南京大学环境学院 [3]湖南省衡南县教师进修学校,湖南衡阳421141

出　　处：《癌变．畸变．突变》2002年第4期210-213,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金项目 (3 9670 15 3 )

摘　　要：目的 :研究两种新型杀虫剂 -吡虫啉和抑食肼对人体外周血淋巴细胞遗传物质的影响。方法 :人体外周血淋巴细胞的微核试验 ,姐妹染色单体互换试验 (SCE)以及单细胞凝胶电泳试验 (SCGE ,又名彗星试验 )。结果 :在低浓度 (吡虫啉为 0 .0 5mg/L ,抑食肼为 5mg/L)时 ,它们对微核和SCE的影响与对照组相比无显著性差异 (P >0 .0 5 ) ,当浓度升高 (吡虫啉为 0 .1mg/L ,抑食肼为 2 5mg/L)时 ,则有显著性差异 (P <0 .0 5 )。而彗星试验在各试验组与对照组相比都有极显著性差异 (P <0 .0 1) ,且存在明显的剂量 -效应关系 (r =0 .995 ,r=0 .96 5 )。结论 :吡虫啉和抑食肼对人外周血淋巴细胞的遗传物质都具有一定程度的损伤作用 ,相比之下 ,吡虫啉比抑食肼具有更大的毒性。Purpose: To study the effects of the two new type pesticides, imidacloprid and RH 5849, on the DNA of human peripheral lymphocytes. Methods: Micronuclei test, SCE test and comet assay were employed in the experiment. Results: At the low dose (imidacloprid 0.05 mg/L and RH 5849 5 mg/L), there were no effect on the micronuclei rate and the SCE rate, compared with the negative controlled groups (P>0.05). But there were adverse effects when the dose increased, and the difference was significant as compared with the negative control groups(P<0.05). In the comet assay, starting from the lowest dose the two pesticides caused DNA damages in the human peripheral lymphocytes as compared with the control groups, and the differences were significant (P<0.01), and there were dose dependent relationship (r=0.995, r=0.965).Conclusion: Both imidacloprid and RH 5849 damaged the DNA of human peripheral lymphocytes. Comparatively. Imidacloprid was more toxic than RH 5849.

关 键 词：吡虫啉 抑食肼 外周血淋巴细胞 遗传物质 姐妹染色单体互换 彗星试验 杀虫剂 

分 类 号：R394[医药卫生—医学遗传学] X172[医药卫生—基础医学]