骨桥蛋白在大肠癌和大肠癌肝转移灶中的表达及其临床意义  

Expression and clinical significance of osteopontin in colorectal cancer and liver metastatic tissues

作　　者：丁凌[1] 郑树[1] 

机构地区：[1]浙江大学肿瘤研究所,杭州310009

出　　处：《中华外科杂志》2002年第10期773-775,I001,共4页Chinese Journal of Surgery

摘　　要：目的　检测骨桥蛋白 (osteopontin ,OPN)mRNA在大肠癌及大肠癌肝转移灶中的表达 ,并探讨其在大肠癌发生、发展及肝转移中的临床意义。　方法　提取 4 4例大肠癌及癌旁正常组织 ,2 0例大肠癌肝转移灶及 3例正常肝组织RNA ,采用RT PCR半定量法检测其OPNmRNA的表达。并用原位杂交行组织定位检测。　结果　 4 4例大肠癌组织OPN表达高于癌旁正常组织 (t =4 89,P =0 0 0 0 )。 4 4例大肠癌组织OPNmRNA表达率为 18 2 % ,而在 2 0例肝转移灶中表达率达 80 0 % (χ2 =2 2 4 2 ,P =0 0 0 0 )。原位杂交表明 ,OPNmRNA定位于大肠癌细胞浆。　结论　OPNmRNA在大肠癌组织中表达高于正常组织 ,而在大肠癌肝转移灶中呈更高表达 ,提示OPN可能是大肠癌肝转移的预测指标之一。Objective To investigate the possible association of osteopontin (OPN) with liver metastasis in colorectal cancer Methods RNA was quantitated by RT PCR from colorectal cancer and surrounding normal tissues, also from liver metastatic tissues and normal liver tissues ISH was utilized to detect OPN mRNA in colorectal cancer and liver metastatic tissues Results In 44 cases, the expressions of OPN mRNA in colorectal cancer tissues were higher than those in the surrounding normal tissues( t =4 89, P =0 000) The expression of OPN mRNA in colorectal cancer was as high as 18 2%, but in liver metastasis was 80%(χ 2=22 42, P =0 000). In ISH, OPN mRNA located in the cytoplasm of colorectal cancer cells. Conclusions The expressions of OPN mRNA were the highest in liver metastatic tissues, moderate in colorectal cancer tissues, and lowest in surrounding normal tissues, indicating that OPN is a potential marker for liver metastasis of colorectal cancer

关 键 词：骨桥蛋白 大肠癌 肝转移 临床意义 RT-PCR半定量法 

分 类 号：R735.34[医药卫生—肿瘤]