T细胞免疫防治BXSB小鼠系统性红斑狼疮的实验研究  

T cell vaccination against systemic lupus erythematosus in BXSB mice

作　　者：李波[1] 韩淑红[1] 刘相国[1] 白晓薇[1] 张岩[1] 高晓明[1] 

机构地区：[1]北京大学医学部免疫学系,北京100083

出　　处：《第二军医大学学报》2002年第10期1091-1094,共4页Academic Journal of Second Military Medical University

基　　金：国家 973计划资助项目 (2 0 0 1CB5 10 0 0 7)

摘　　要：目的 :探索用自身反应性 T细胞免疫防治 BXSB雄性小鼠发生类似人系统性红斑狼疮 (SL E)的可行性。方法 :用活化的 BXSB小鼠自身反应性 T细胞克隆 (ATL 1)从小鼠尾静脉注射进行免疫 (1× 10 6 /只 ,共 3次 ) ,然后从不同角度监测病情发展情况。 结果 :在免疫后的第 1、2、4周 ,T细胞免疫组小鼠血清中抗 ds DNA抗体水平明显低于对照组 (P<0 .0 1) ,在免疫后第 4、8、12、16周 ,T细胞免疫组小鼠的死亡率明显低于对照组 (P<0 .0 5 ) ,但是两组尿蛋白的浓度在各时间段均未见显著差异。结论 :T细胞免疫能在一定程度上延缓 BXSB小鼠 SL E的进程 ,延长小鼠的生存时间。其机制可能与 T细胞免疫诱导了针对自身反应性 T细胞的调节性免疫应答有关。Objective: To investigate the feasibility of treating murine lupus by vaccination with autoreactive T cells (ATL1, derived from BXSB mice with murine lupus). Methods: Young male BXSB mice were injected intravenously with activated ATL1 cells(1×10 6/mouse) 3 times in total. Results: In the 1st, 2nd and 4th weeks of T cell vaccination, the levels of serum autoantibodies against dsDNA in the immunized group were significantly lower compared with that of control group ( P < 0.01). In the 4th, 8th, 12th and 16th weeks after immunization, the mortality of the former group was also lower than that of control group ( P <0.05), but there was no significant difference between their urine protein concentration. Conclusion: T cell vaccination can postpone the onset of SLE in BXSB mice and prolong the survival of disease animals. The possible mechanisms may be that T cells induce regulatory immuno activity targeting autoimmune T cells.

关 键 词：T细胞免疫 防治 BXSB小鼠 系统性红斑狼疮 实验研究 

分 类 号：R593.241[医药卫生—内科学]