电位滴定法测定胶原的表观解离平衡常数  

Determination of apparent dissociation constant of collagen by potentiometric method

作　　者：王力[1,2] 周继博 张文华[2] WANG Li;ZHOU Ji-bo;ZHANG Wen-hua(The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education,Sichuan University,Chengdu 610065,China;National Engineering Laboratory for Clean Technology of Leather Manufacture,Sichuan University,Chengdu 610065,China)

机构地区：[1]四川大学皮革化学与工程教育部重点实验室,四川成都610065 [2]四川大学制革清洁技术国家工程实验室,四川成都610065

出　　处：《化学研究与应用》2016年第8期1141-1144,共4页Chemical Research and Application

基　　金：国家高技术研究发展计划项目(2013AA06A306)资助

摘　　要：论文采用电位滴定法测定不同离子强度下胶原蛋白的表观解离平衡常数,根据Katchalsk-Spitnik方程作图和计算。结果表明:随着离子强度的增大,胶原的表观解离常数随之减小,其值分别为:7.94(0 mol·L-1Na Cl)、7.90(0.1 mol·L-1Na Cl)、7.90(0.2 mol·L-1Na Cl)和7.88(0.4 mol·L-1Na Cl),离子强度对胶原的表观解离常数影响较小。The apparent dissociation constants of collagen under different ionic strength were determined by potentiometric titration,and the data were analyzed based on Katchalsk-Spitnik equation. The results indicate that collagen’s apparent dissociation constantdecreases slightly with the increase of ionic strength,and the value is 7. 94(0 mol·L-1 NaCl),7. 90(0. 1 mol·L-1 NaCl),and 7. 90(0. 2 mol·L-1 NaCl),7. 88(0. 4 mol·L-1 NaCl)respectively. The ionic strength has a weak influence on apparent dissociation constantsof collagen.

关 键 词：胶原 电位滴定 解离常数 离子强度 

分 类 号：O657.1[理学—分析化学]