EB病毒感染用两种检测方法结果分析  

Analysis of the results of two detection methods for EB virus infection

作　　者：赵凯丽[1] 周贺[1] 王芳[1] 林牧[1] 龚亚东[1] Zhao Kaili;Zhou He;Wang Fang;Lin Mu;Gong Yadong(Department of Pediatrics,Guizhou Aerospace Hospital 563000)

机构地区：[1]贵州航天医院儿科,563000

出　　处：《中国社区医师》2016年第30期127-128,共2页Chinese Community Doctors

摘　　要：目的:探讨ELISA法和QF-PCR法在诊断儿童EB病毒感染中的临床意义。方法:采用ELISA法和QF-PCR法检测150例疑似EBV感染患儿EBV-IgM抗体与EBV-DNA,并对检测数据进行统计学分析。结果:150例受检感染患儿中,ELISA法检测EBV-IgM抗体检出阳性50例(33.33%),QF-PCR法检测咽拭子样本EBV-DNA阳性78例(52.00%),QF-PCR法检测全血样本EBV-DNA阳性69例(46.00%),两组数据间比较,差异具有统计学意义(P<0.01或P<0.05)。≤7d血清EBV-IgM与QF-PCR全血标本检测阳性结果比较,差异具有统计学意义(P<0.05)。结论:血清学检查和QF-PCR法检测EB病毒符合率是一致的,且FQ-PCR灵敏度更高,联合检测更有意义。Objective:To explore the clinical significance of ELISA and QF-PCR methods in the diagnosis of EB virus infectionin children.Methods:Using of ELISA method and QF-PCR method to detect the EBV-IgM antibody and EBV-DNA in 150children with suspected EBV infection,and then the data were statistically analyzed.Results:Among those 150 children withinfection,50 cases(33.33% ) were detected as positive EBV-IgM antibody by ELISA method;78 cases(52% ) with positiveEBV-DNA of throat swab specimens detected by QF-PCR;69 cases(46%) with EBV-DNA positive of blood samples detected byQF-PCR;the differences were statistically significant between groups(P<0.01 or P<0.05).If the course of disease less than 7days,the difference in positive result between serum EBV-IgM and QF-PCR blood samples was statistically significant(P<0.05).Conclusion:The coincidence rate in serological examination and QF-PCR detection of EB virus is consistent,and FQ-PCRsensitivity is higher.It is more meaningful to joint detection methods.

关 键 词：EB病毒感染 ELISA QF-PCR 

分 类 号：R725.1[医药卫生—儿科]